Gsh kit

Manufactured by Biodiagnostic

Sourced in Egypt

The GSH kit is a laboratory equipment used for the quantitative determination of glutathione (GSH) levels in various biological samples. It provides a reliable and standardized method for measuring this important antioxidant compound.

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Lab products found in correlation

Creatinine, by Biodiagnostic (1 mentions) Mtt assay kit, by Abcam (1 mentions) Bcl 2 elisa kit, by Zymo Research (1 mentions) Urea kits, by Biodiagnostic (1 mentions) Mda kit, by Biodiagnostic (1 mentions) Pierce bicinchoninic acid bca assay kit, by Thermo Fisher Scientific (1 mentions)

3 protocols using gsh kit

Biochemical Assays for Cell Viability and Apoptosis

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MTT assay kit and propidium iodide (PI) staining FC assay kit was purchased from Abcam (Boston, MA, USA). AST, ALT, and bilirubin (total and direct) kits and creatinine, urea kits, and GSH kit were all purchased from Biodiagnostics Company (Cairo, Egypt).
Heparanase ELISA kit (Abcam, Cambridge, UK), Annexin V/PI double staining kit (eBiosciencesDx), EGFR 96-well plate kit (Abcam, Cambridge, UK), uPA ELISA kit (Creative Diagnostics, New York, NY, USA), and BcL-2 ELISA kit (Zymed Laboratories, Carlsbad, CA, USA) were used.

Eldeeb M., Sanad E.F., Ragab A., Ammar Y.A., Mahmoud K., Ali M.M, & Hamdy N.M. (2022). Anticancer Effects with Molecular Docking Confirmation of Newly Synthesized Isatin Sulfonamide Molecular Hybrid Derivatives against Hepatic Cancer Cell Lines. Biomedicines, 10(3), 722.

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Hepatic Oxidative Stress Biomarkers Quantification

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Hepatic levels of malondialdehyde (MDA), a marker of lipid peroxidation, were determined by a thiobarbituric acid method using MDA kit (Biodiagnostic, Egypt). The formed colored thiobarbituric acid reactive product was measured spectrophotometrically at 534 nm (Gavino et al., 1981 (link)). Hepatic glutathione (GSH) levels were measured by GSH kit (Biodiagnostic, Egypt) based on the reduction of 5,5‘dithiobis (2-nitrobenzoic acid) by GSH. The absorbance of colored product was measured at 405 nm (Beutler, 1963 (link)). Aliquots of liver homogenate were used for determination of nuclear Nrf-2, GSK-3β and PI3K levels using rat-specific ELISA kits provided by Wuhan Fine Biological Technology Co., China (catalog no.: ER911, ER0124 and ER1531, respectively). Hepatic levels of HO-1 were estimated by rat-specific ELISA kit supplied by Elabscience Biotechnology Inc., USA (catalog no.: E-EL-R0864) while hepatic rat pAkt concentrations were measured by Cusabio® ELISA kit (China). The Pierce™ bicinchoninic acid (BCA) assay kit supplied by ThermoFisher Scientific, USA (catalog no.: 23227) was used to determine total protein content (Smith et al., 1985 (link)). Tissue parameters were expressed per mg of protein.

Saleh D.O., Mahmoud S.S., Hassan A, & Sanad E.F. (2022). Doxorubicin-induced hepatic toxicity in rats: Mechanistic protective role of Omega-3 fatty acids through Nrf2/HO-1 activation and PI3K/Akt/GSK-3β axis modulation. Saudi Journal of Biological Sciences, 29(7), 103308.

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Colorimetric Assay for GSH Levels

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GSH brain levels were assayed using GSH kit of Biodiagnostic, Egypt. The method, according to Beutler and Kelly, depends on the reduction of 5,5’-dithiobis-(2-nitrobenzoic acid) (DTNB) by the SH group in glutathione (GSH) to produce an intense yellow compound. The reduced chromogen is directly proportional to GSH concentration and its absorbance can be measured colorimetrically at 405 nm [81 (link)].

Thabit S., Handoussa H., Roxo M., Cestari de Azevedo B., S.E. El Sayed N, & Wink M. (2019). Styphnolobium japonicum (L.) Schott Fruits Increase Stress Resistance and Exert Antioxidant Properties in Caenorhabditis elegans and Mouse Models. Molecules, 24(14), 2633.

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