Sections from paraffin embedded livers or muscle cryosections collected on slides were stained with Modified Mayer’s Hematoxylin (Sigma) for 2 min, and with Eosin Y (Sigma) for 1 min. Muscle cryosections were fixed in cold 4% PFA for 20 min on ice prior to H&E staining. Between stains tissue was washed as follows: 2x rinse in distilled water, 10% acetic acid for 1 min, 2x rinse in distilled water, Scotts Tap Water for 2 min, rinse in distilled water and 70% ethanol for 1 min. Stained tissue was rinsed in 90% ethanol, serially dehydrated in 90%, 95%, and 100% ethanol, incubated twice in xylene for 2 min and mounted with Permount Mounting Medium (Electron Microscopy Sciences). Sections from paraffin embedded livers were serially deparaffinized before staining.
Modified mayer s hematoxylin
Manufactured by Merck Group
Sourced in Germany
Modified Mayer's Hematoxylin is a laboratory reagent used for the staining of nuclei in histological and cytological specimens. It is a modified version of the original Mayer's Hematoxylin formulation. The product is designed to provide clear nuclear staining in various tissue samples.
Automatically generated - may contain errors
Lab products found in correlation
Permount mounting medium, by Electron Microscopy Sciences (2 mentions)
Eosin y, by Merck Group (2 mentions)
Microscope slide, by Matsunami (1 mentions)
Eosin y, by Fujifilm (1 mentions)
Avidin biotin peroxidase complex, by Merck Group (1 mentions)
Goat anti rabbit igg, by Merck Group (1 mentions)
Anti ucp1, by Merck Group (1 mentions)
4 protocols using modified mayer s hematoxylin
1
Histological Staining of Tissue Sections
2
Histological Staining of Tissue Sections
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Sections from paraffin embedded livers or muscle cryosections collected on slides were stained with Modified Mayer’s Hematoxylin (Sigma) for 2 min, and with Eosin Y (Sigma) for 1 min. Muscle cryosections were fixed in cold 4% PFA for 20 min on ice prior to H&E staining. Between stains tissue was washed as follows: 2x rinse in distilled water, 10% acetic acid for 1 min, 2x rinse in distilled water, Scotts Tap Water for 2 min, rinse in distilled water and 70% ethanol for 1 min. Stained tissue was rinsed in 90% ethanol, serially dehydrated in 90%, 95%, and 100% ethanol, incubated twice in xylene for 2 min and mounted with Permount Mounting Medium (Electron Microscopy Sciences). Sections from paraffin embedded livers were serially deparaffinized before staining.
3
Histological Analysis of Tissue Samples
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Histological analysis was performed as described in previous studies (48 (link), 49 (link)) with some modifications. Briefly, tissues were excised from each mouse and fixed in 4% (v/v) paraformaldehyde/PBS. After ethanol dehydration, the fixed samples were embedded in paraffin, cut into 5 μm sections with a microtome, and mounted on microscope slides (Matsunami Glass). The sections were stained with modified Mayer’s hematoxylin (Merck) and eosin Y (Wako Pure Chemical Industries Ltd). For immunohistochemistry analysis, the sections were incubated in 1% (v/v) hydrogen peroxide in methanol and treated with 10% (v/v) normal goat serum, rabbit anti-UCP1 (U6382; 1:200; Sigma–Aldrich), goat anti-rabbit IgG (Nichirei), and avidin-biotin-peroxidase complex (Nichirei).
4
Immunohistochemical Analysis of UCP1 in Adipose Tissues
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The interscapular BAT, and inguinal WAT, were fixed in 4% paraformaldehyde and embedded in paraffin. For UCP1 immunohistochemistry, paraffin-embedded sections (6μm) were incubated with anti-UCP1 (Sigma), followed by detection using the ABC62 (link) method. Nuclei were counterstained with modified Mayer’s hematoxylin (Merck, Darmstadt, Germany)62 (link).
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