Alexa fluor 488 annexin 5 dead cell apoptosis kit

Regorafenib was purchased from MedChemExpress (New Jersey, USA). Staurosporine (STS), N-acetyl-l-cysteine (NAC), ROS assay Kit, JC-1 assay Kit, and caspase-3/8/9 activity assay Kits were obtained from Beyotime (Shanghai, China). Rabbit monoclonal antibodies to PI3K (4292), Bim (2933), Bax (5023), Bak (12105), Puma (98672), Bad (9268), Bcl-xl (2764), p-AKT (Ser473) (4060), AKT (9272), FOXO3a (12829), Drp1 (8570), Mff (14739), MFN1 (14739), MFN2 (11925), and PARP (9532) antibodies were purchased from Cell Signaling Technology (Massachusetts, USA). Luciferase Mycoplasma Detection Kit, mouse monoclonal antibodies tubulin, actin and GAPDH antibodies were obtained from TransGen (Beijing, China). TurbofectTM transfection reagent and Mitotracker Deep Red 633 were obtained from Thermo Fisher Scientific (Massachusetts, USA). Cell counting Kit-8 (CCK-8) and Alexa Fluor® 488 annexin V/Dead Cell Apoptosis Kit were obtained from Dojindo (Kyushu, Japan). Hoechst 33258 probe and PI probe were purchased from Sigma-Aldrich (Missouri, USA). Enhanced chemiluminescence (ECL) was obtained from Biosharp (Beijing, China).

Met was purchased from Solarbio (Beijing, China). Staurosporine (STS), Caspase-3/8/9 Assay Kit, ROS Assay Kit and JC-1 Assay Kit were provided by Beyotime (Shanghai, China). Anti-PARP (9532), anti-Bak (12105), anti-Bax (5023), anti-Bcl-xL (2764), anti-Mcl-1 (94296), anti-Bim (2933), anti-Bad (9268), anti-Puma (98672), anti-AMPK (5831), anti-p-AMPK (8208), anti-Cleaved Caspase-3 (9664), anti-Cleaved Caspase-8 (8592), anti-Caspase-9 (9502) Rabbit monoclonal antibody, and anti-Bid (8762) Mouse monoclonal antibody were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-GAPDH (sc-47724) Mouse monoclonal antibody was obtained from Santa Cruz (Texas, USA). Luciferase Mycoplasma Detection Kit and anti-β-tubulin (HC101) Mouse monoclonal antibody were purchased from Transgen Biotech (Beijing, China). Turbofect™ transfection regent and Mito-tracker Deep Red dye were obtained from ThermoFisher Scientific (Massachusetts, USA). Cell counting Kit-8 (CCK-8) and Alexa Fluor^® 488 annexin V/Dead Cell Apoptosis Kit were purchased from Dojindo (Kyushu, Japan). Acetylcysteien (NAC), Z-VAD-(OMe)-FMK (Z-VAD) and Compound C were purchased from MedChemExpress (New Jersey, USA). Hoechst 33258 dye and PI dye were obtained from G-clone (Beijing, China).