Genomic DNA was isolated from peripheral blood samples by using a FUJI whole blood DNA kit (Fujifilm Corporation, Japan). The three SNPs (rs738722, rs2074356, and rs2274223) were genotyped by a Taqman real-time polymerase chain reaction method using a 7900 HT sequence detector system (Applied Biosystems, Foster City, CA, USA). Probes for these three SNPs were ordered from Applied Biosystems. Allelic discrimination was measured automatically by Sequence Detection Systems 2.3 software (Applied Biosystems). For each SNP, more than 98% of the samples were genotyped successfully. To verify the reproducibility of the Taqman genotyping results, 10% of the samples were randomly selected and retested. The concordance was 99%.
Fuji whole blood dna kit
Manufactured by Fujifilm
Sourced in Japan
The FUJI whole blood DNA kit is a laboratory equipment product designed for the extraction and purification of genomic DNA from whole blood samples. It provides a reliable and efficient method for obtaining high-quality DNA suitable for various downstream molecular biology applications.
Automatically generated - may contain errors
2 protocols using fuji whole blood dna kit
1
Genotyping of Three SNPs in Blood
2
Genotyping of Pharmacogenomic Variants
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We searched the National Center for Biotechnology Information (NCBI) SNP database (dbSNP; http://www.ncbi.nlm.nih.gov/snp/ ) and related literature to identify mSNPs from the DPD, TP, TS and ENOSF1 genes. The criteria for SNP selection were as follows: (1) With a MAF of more than 0.10 in Asian population, (2) Genotypes call rate ≥ 95%, (3) Missense SNP. Three SNPs (DPD rs1801159; TP rs11479 and ENOSF1 rs2612086) were selected for genotyping. None SNP in TS gene met the inclusion criteria. Genomic DNA was extracted from peripheral blood using a FUJI whole blood DNA kit (Fujifilm Corporation, Tokyo, Japan). Primers were designed by Genotyping Tools and MassARRAY Assay Design software (version 3.0, Sequenom Inc., San Diego, California). SNPs were genotyped using the Sequenom MassARRAY iPLEX platform. Data were processed and analyzed by Sequenom MassArray TYPER 4.0 software. Details of PCR reactions are in supplementary file . Primer sequences are presented in Supplementary Table S5 . Five percent of the samples were randomly selected and genotyped by direct sequencing, with a resulting concordance rate of 100%. Call rate threshold was set at least 95% for each SNP. HWE was tested through χ2 test and p < 0.05 indicated deviation from the equilibrium.
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