Pkr b 10

Manufactured by Santa Cruz Biotechnology

The PKR B-10 is a laboratory equipment designed for the detection and analysis of protein kinase R (PKR) activity. It provides a reliable and efficient method for researchers to study the involvement of PKR in various cellular processes and disease states.

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Lab products found in correlation

Phospho t446 pkr, by Abcam (1 mentions) Penta his, by Qiagen (1 mentions) Phospho eif2α ser51, by Cell Signaling Technology (1 mentions) Eif2α l57a5, by Cell Signaling Technology (1 mentions) Pkr d7f7, by Cell Signaling Technology (1 mentions) Western star chemiluminescent detection system, by Thermo Fisher Scientific (1 mentions) Pvdf membrane, by Merck Group (1 mentions) β actin, by Santa Cruz Biotechnology (1 mentions)

2 protocols using pkr b 10

Western Blot Immunodetection Protocol

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With the exception of pulldown assays (above), lysates for immunoblot assays were prepared by washing the monolayer with phosphate-buffered saline and lysing the cells with 2% SDS. After sonication to disrupt the cellular DNA, proteins were separated by 10% SDS-PAGE, transferred to a PVDF membrane (Millipore), and probed with the indicated antibodies using the Western Star chemiluminescent detection system (Applied Biosystems). Antibodies used in these experiments include eIF2α L57A5 (#2103), phospho-eIF2α Ser51 (#3597), and PKR D7F7 (#12297), all from Cell Signaling Technology, PKR B-10 (sc6282, Santa Cruz Biotechnology), Penta-His (Qiagen), phospho-PKR T446 (ab32026, AbCam), and Actin (A2066, Sigma). Rabbit antiserum that recognizes the TRS1/IRS1 dsRNA binding domain (anti-p999) has been described previously (Marshall et al., 2009 (link)).

Braggin J.E., Child S.J, & Geballe A.P. (2015). Essential Role of Protein Kinase R Antagonism by TRS1 in Human Cytomegalovirus Replication. Virology, 489, 75-85.

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Immunoblot Analysis of Phosphorylated Proteins

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At 48 or 72 h after transfection, the cell extracts were prepared and immunoblot assays performed as described elsewhere [25 (link)–27 (link)]. The following antibodies were used: PKR (B-10), PKR (K-17) and beta-actin (Santa Cruz Biotechnology, Santa Cruz, CA); phospho-PKR [pT451] and phospho-eIF-2α [pS51] (BioSource International, Camarillo, CA).

Hao C., Shao R., Raju U., Fang B., Swisher S.G, & Pataer A. (2016). Accumulation of RNA-dependent protein kinase (PKR) in the nuclei of lung cancer cells mediates radiation resistance. Oncotarget, 7(25), 38235-38242.

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