Cytolyt fluid

After anonymization of urine samples, they were stored at 4 °C, then centrifuged for 5 min on a Sorvall ST 16 Centrifuge (Thermo Scientific) at 1500 r.p.m. The pelleted cell deposits were inspected and the supernatant was disposed. When deposits weren’t visible, a vial of PreservCyt fluid (Hologic) was placed into the Universal container, then the contents were mixed and tipped back into the PreservCyt vial.
When the deposit amount was large but not bloodstained abundantly, the deposit was stirred using a Pasteur pipette, then three drops were applied to the PreservCyt vial. When the deposit was abundantly stained with blood, 15 mL of CytoLyt fluid (Hologic) was added to lyse the blood in the deposit, then centrifuged for 10 min at 1500 r.p.m. then 3 drops were applied to the PreservCyt vial.
Using non-gynecological blue filters, samples were processed on an automated ThimPrep 2000 processor (Hologic). A ThinPrep slide with a monolayer of cells is the result of this process. Then, the slides were fixed with Cytofix spray (Hologic) and allowed to dry for 15 min. Sample slides were stored between 2 and 8 °C and stained in weekly batches using URO17® (Lot: A8034124, 3.42 mg/mL, 1:5000 dilution in 10% CS) on a Leica detection kit DS9800 for 20 min.

Urine samples were anonymized, stored at 4°C and underwent centrifugation on a Sorvall ST 16 Centrifuge (Thermo Scientific) at 1500 r.p.m. for 5 minutes. The supernatant was discarded, and the pelleted cell deposits were then inspected. If the deposit was hardly visible, a vial of PreservCyt fluid (Hologic) was added into the Universal container, the contents mixed and tipped back into the PreservCyt vial. If the deposit was large but not excessively bloodstained, then, the deposit was mixed using a Pasteur pipette, three drops were added to the PreservCyt vial. If the deposit was excessively blood stained, then, 15 mL of CytoLyt fluid (Hologic) was added to the deposit to wash the sample (this lyses the blood) and the sample underwent centrifugation for 10 minutes at 1500 rpm and three drops of the deposit was added to a PreservCyt vial.
Samples were processed on an automated ThinPrep 2000 processor (Hologic) using non‐gynecological blue filters. This processor generates a ThinPrep slide with a monolayer of cells. Slides were then fixed with Cytofix spray (Hologic) and allowed to dry for 15 minutes.
Sample slides were stored between 2‐8°C and stained in weekly batches using URO17™ (Lot: A8034124, 3.42 mg/mL, 1:5000 dilution in 10% CS) on a Leica detection kit DS9800 for 20 minutes.