We will target either candidate gene variants [48 (link),49 (link)] or Genome-Wide Association–based variants previously related to skeletal muscle and bone health [50 (link),51 (link)]. Genomic DNA will be extracted from residual blood samples from Becton Dickinson (BD) Vacutainer EDTA tubes using the MagSep Blood gDNA kit (0030 451.00, Eppendorf) or GeneJET Genomic Whole Blood DNA Purification Kit (#K0781 Thermo Scientific). Gene variants will be determined using the TaqMan SNP assay (Applied Biosystems, Thermo Fisher Scientific) by QuantStudio 7 Flex (Applied Biosystems, Thermo Fisher Scientific). Genotyping will be replicated in another independent institute, as previously described [52 (link),53 (link)], to validate the results.
Magsep blood gdna kit
Manufactured by Eppendorf
Sourced in Germany
The MagSep Blood gDNA kit is a laboratory equipment product designed for the extraction and purification of genomic DNA from whole blood samples. It utilizes magnetic bead-based technology to capture and isolate the DNA, providing a reliable and efficient method for DNA extraction.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using magsep blood gdna kit
1
Validating Genetic Variants Linked to Muscle and Bone
2
Genetic Risk Score Calculation for BMD
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Genomic DNA was extracted from residual blood samples from BD Vacutainer EDTA tubes using the MagSep Blood gDNA kit (0030 451.00, Eppendorf, Hamburg, Germany) [26] . The genotype of each SNP was assessed by the Australian Genome Research Facility (AGRF). We chose the SNPs based on previous literature from a GWAS study assessing BMD [16] . The SNPs, locus, type, effect allele and closest gene are described in supplementary table 1. Based on the Moayyeri et al GWAS, nine genetic variants known to be associated with broadband ultrasound attenuation (BUA), that estimates BMD, were used to calculate genetic risk scores (GRS).The SNPs were measured by MassARRAY® combined with iPLEX® chemistry (Agena Bioscience).
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