Four cell lines (human colon cancer cell line HCT116, human embryonic kidney cell line HEK293, human liver cancer cell line HepG2, and human B lymphoma cell line SU-DHL-6), fetal bovine serum (FBS), Eagle's Minimum Essential Medium (EMEM), and Roswell Park Memorial Institute (RPMI) 1640 medium were purchased from American Type Cell Collection (ATCC, Manassas, VA, USA). H9-derived human neural stem cells, defined FBS, trypsin-EDTA, fibronectin, basal fibroblast growth factor (bFGF), epidermal growth factor (EGF), KnockOut™ Dulbecco's Modified Eagle Medium (DMEM)/F12, and penicillin-streptomycin, StemPro® Neural Supplement were acquired from Life Technologies, Carlsbad, CA, USA. Chemicals and epigenetic compound libraries were purchased from Cayman Chemicals (Ann Arbor, MI), Santa Cruz Biotechnology (Dallas, TX, USA), SelleckChem (Houston, TX, USA), and Sigma-Aldrich (St. Louise, MO, USA). HDAC-Glo I/II and CellTiter-Glo reagents were purchased from Promega, Madison, WI. Fluorogenic assay kits for HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, and HDAC10 were acquired from BPS Bioscience (San Diego, CA). White solid bottom 1536-well assay plates were purchased from Greiner Bio-One (Monroe, NC).
Hdac7
Manufactured by BPS Biosciences
Sourced in United States
HDAC7 is a recombinant protein produced by BPS Biosciences. It is a member of the histone deacetylase (HDAC) family of enzymes, which are responsible for the removal of acetyl groups from histones, leading to changes in chromatin structure and gene expression.
Automatically generated - may contain errors
Lab products found in correlation
Hdac9, by BPS Biosciences (2 mentions)
Hdac5, by BPS Biosciences (2 mentions)
Hdac2, by BPS Biosciences (2 mentions)
Hdac6, by BPS Biosciences (2 mentions)
Hdac4, by BPS Biosciences (2 mentions)
Hdac3, by BPS Biosciences (2 mentions)
Hdac8, by BPS Biosciences (2 mentions)
Epidermal growth factor (egf), by Thermo Fisher Scientific (1 mentions)
Celltiter glo reagent, by Promega (1 mentions)
Fibronectin, by Thermo Fisher Scientific (1 mentions)
Stempro neural supplement, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Hepg2, by American Type Culture Collection (1 mentions)
Hct116, by American Type Culture Collection (1 mentions)
Hdac1, by BPS Biosciences (1 mentions)
2 protocols using hdac7
1
High-Throughput Screening of Epigenetic Modulators
2
HDAC Enzyme Activity Assay Protocol
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Enzyme activity measurements of class I and IIa HDACs were performed using a cell‐free system and according to a method previously described.16 We used fluorogenic HDAC1 (#50061), HDAC2 (#50062), HDAC3 (#50063), HDAC4 (#50064), HDAC5 (#50065), HDAC6 (#50076), HDAC7 (#50067), HDAC8 (#50068) and HDAC9 (#50069) enzyme assay kits from BPS Bioscience (San Diego, CA, USA). To test the enzyme activity of LMK235, 0.001, 0.003, 0.01, 0.03, 0.1, 0.3, 1, 3 and 10 μmol/L concentrations were used. For the IC50 calculations, every data point was normalized to the vehicle (100% activity). The normalized data were fitted with a Hill non‐linear curve fit (OrigionPro 9.0). Employing the “Find X from Y” function in OrigionPro 9.0 resulted in the IC50 values (50% activity).
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