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3 protocols using ir 808

1

Photosensitizer-Mediated Cancer Therapy

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Copper(II) nitrate hydrate (Cu(NO3)2·3H2O), 2,5-dimercaptoterephthalic acid (DCA), IR 783, IR 808, Chlorin e6 (Ce6), Indocyanine green (ICG), Tris(2,2-bipyridine) ruthenium chloride (RDPP), H2O2 (technical grade, 30%) were provided by Sigma–Aldrich (USA). Nitrotetrazolium blue chloride (NBT) were purchased from TCI (Japan). The CCK-8 kit was purchasedfrom MCE (USA). A Tris-HCl (pH = 8.8, 1 M), anhydrous (NaCO3), BCA protein concentration determination kit, Calcein/PI cell viability/cytotoxicity assay kit, reactive oxygen detection kit, crystal violet were obtained from Beyotime (China). Matrigel® Matrix was purchased from Corning (USA). Penicillin–streptomycin solution (100X), foetal bovine serum (FBS), trypsin, Dulbecco’s modified Eagle’s447 medium (DMEM) were obtained from Gibco (USA). Phosphate-buffered saline (PBS, pH = 7.4) solution was prepared in the laboratory. All chemicals and reagents were of analytical grade and used as received without further purification. Millipore deionized water (18.2 MΩ · cm) was used for all experiments.
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2

Heptamethine Cyanine Dyes for Glioblastoma

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Heptamethine cyanine dyes (IR-775, IR-780, IR-783, IR-797, IR-806, IR-808), Intralipid and dimethyl sulfoxide (DMSO) were obtained from Sigma-Aldrich (St. Louis., MO, USA). Cell counting kit-8 (CCK-8) was purchased from Dojindo (Tokyo, Japan). Calcein-AM and propidium iodide (PI), fetal bovine serum (FBS), DMEM, trypsin-EDTA and penicillin-streptomycin were purchased from Gibco Life Technologies (AG, Switzerland). All other chemicals used in this study were of analytical reagent grade and used without further purification. Milli-Q grade (R > 18 MΩ cm) water was used throughout. Human glioblastoma luciferase-labeled U87 cell lines (U87L) were purchased from American Type Culture Collection (ATCC, Manassas, VA).
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3

Multifunctional Nanoparticle Synthesis and Applications

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Cerium (III) nitrate hexahydrate (Ce (NO3)3·6H2O), glucose (Glu), curcumin, alendronate (AL), Tris (2,2-bipyridine) ruthenium chloride (Ru(ddp)), 2-methylimidazole (HMIM), IR780, IR783, IR808, Ce6, Cy3, copper nitrate trihydrate (Cu(NO3)2·3H2O), manganese dichloride (MnCl2), magnesium chloride (MgCl2), cobalt chloride (CoCl2), iron trichloride (FeCl3) and streptozotocin (STZ) were provided by Sigma–Aldrich (USA). Chlorin e6 (Ce6) was obtained from Frontier (UK). glucose oxidase (GOx) and nitrotetrazolium blue chloride (NBT) were purchased from TCI (Japan). Methyl red, 2′,7′-dichlorofluorescin diacetate (DCFH-DA), hematoxylin-eosin (H&E), Masson's trichrome, Sirius staining solution and dihydroethidium (DHE) were purchased from Beyotime Biotechnology (China). Penicillin–streptomycin solution (100X), fetal bovine serum (FBS), trypsin and Dulbecco's modified Eagle's medium (DMEM) and RPMI-1640 medium (1640) were obtained from Gibco (USA). The Cell Counting Kit-8(CCK-8 kit) was purchased from MCE (USA). Anti-CD31 antibody, anti-α-SMA antibody and goat anti-rabbit IgG secondary antibody were purchased from Abcam (UK). High-purity (Milli-Q) water with a resistivity of 18.2 ​MΩ ​cm was obtained from an inline Millipore RiOs/Origin water purification system.
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