Anti fgl1

The experimental procedure was performed as described in the previous study [32 (link)]. The total protein concentration of cells and tissues was measured using the BCA protein quantification method (Beyotime, China). Equal amounts of proteins were resolved by electrophoresis on 10% SDS gel and then transferred onto nitrocellulose membranes. Following blocked by incubation in 5% nonfat milk, membranes were probed with specific anti-CD9 (Cell Signaling Technology, 1:1000), anti- CD63 (Cell Signaling Technology, 1:1000), anti- CD81 (Cell Signaling Technology, 1:1000), anti- FGL1 (Abcam, 1:1500), anti-p-p65 (Abcam, 1:1500), anti-p65 (Abcam, 1:1000), anti-IκBα (Abcam, 1:1000), anti-p-IκBα (Abcam, 1:1500), anti-Bcl-2 (Abcam, 1:1500), anti-Bax (Abcam, 1:1500) and anti-cleaved-caspase 3 (Abcam, 1:1000) overnight at 4°C. After washing with Tris buffer saline (TBS) containing 0.24% Tween-20, membranes were then incubated for 60 min with horseradish peroxidase-conjugated secondary antibody. Then, an enhanced chemiluminescence system was used for visualization of protein signals, and the density of each protein band was analyzed using Image-Pro Plus6.0 (Media Cybernetics, Silver Spring, USA).