Moor ldi 5061

To induce peripheral arteriogenesis, C57/BL6 mice underwent unilateral femoral artery ligation (occ) of the right leg whereas the left leg was sham operated as previously described. 5, 20 During surgery none of the mice died and no necrosis or infection of the wound was observed during the observation period.
Blood flow measurements were conducted using the LDI technique (Moor LDI 5061 and Moor Software Version 3.01, from Moor Instruments, Remagen, Germany) as previously described. 5, 20 Mice were anesthetized and kept at 37 °C body temperature in a heating chamber for 10 min. The measurements were performed before surgery, directly after surgery, and on day 3 and day 7 after surgery. The right-to-left ratio was calculated for each mouse and background tissue values were subtracted to eliminate tissue bias.
Blood pressure was measured non-invasively by determining the tail blood pressure using a two-module system consisting of an occlusion tail cuff and a volume pressure recording sensor from CODA System (Kent Scientific, Torrington, CT, USA). The measurements were done as previously described. 21 The blood pressure was measured one day after the surgical procedure and at day 7.