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Nucleobond xtra maxi plus ef kit

Manufactured by Macherey-Nagel
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The NucleoBondTM Xtra Maxi Plus EF kit is a laboratory product designed for the purification of high-quality plasmid DNA. It utilizes a modified alkaline lysis procedure and a patented anion-exchange resin to efficiently isolate plasmid DNA from bacterial cultures.

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Lab products found in correlation

Dna spin plasmid dna purification kit, by iNtRON Biotechnology (2 mentions) Nucleobond xtra midi plus ef kit, by Macherey-Nagel (1 mentions) Endofree plasmid giga kit, by Qiagen (1 mentions)

4 protocols using nucleobond xtra maxi plus ef kit

1

Plasmid DNA Extraction from E. coli

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Plasmid DNA from E. coli was prepared using a DNA Spin Plasmid DNA purification Kit (Intron Biotechnology, Korea) or a NucleoBondTM Xtra Maxi Plus EF kit (Macherey-Nagel, Germany). General genetic engineering procedures were performed as described by Sambrook and Russell [68 ].
Elias A., Gritsenko N., Gorovits R., Spector I., Prag G., Yagil E, & Kolot M. (2018). Anti-cancer binary system activated by bacteriophage HK022 integrase. Oncotarget, 9(44), 27487-27501.
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2

Plasmid DNA Purification from E. coli

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Plasmid DNA from E. coli was prepared using a DNA Spin Plasmid DNA purification Kit (Intron Biotechnology, Korea) or a NucleoBondTM Xtra Maxi Plus EF kit (Macherey-Nagel, Germany). General genetic engineering experiments were performed as described by Sambrook and Russell37 .
Elias A., Spector I., Sogolovsky-Bard I., Gritsenko N., Rask L., Mainbakh Y., Zilberstein Y., Yagil E, & Kolot M. (2016). Cancer-specific binary expression system activated in mice by bacteriophage HK022 Integrase. Scientific Reports, 6, 24971.
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3

Plasmid DNA Isolation for Experiments

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pTA22-YFP was isolated using the MACHEREY NAGEL NucleoBond Xtra Maxi Plus EF kit (740426.50). All other plasmids were isolated using the MACHEREY NAGEL NucleoBond Xtra Midi Plus EF kit (740422.50). The pooled library was isolated using the QIAGEN EndoFree Plasmid Giga kit (12391). Isolated plasmid DNA was resuspended at a concentration of 1 µg μl−1 (measured on NanoDrop).
Arndell T., Chen J., Sperschneider J., Upadhyaya N.M., Blundell C., Niesner N., Outram M.A., Wang A., Swain S., Luo M., Ayliffe M.A., Figueroa M., Vanhercke T, & Dodds P.N. (2024). Pooled effector library screening in protoplasts rapidly identifies novel Avr genes. Nature Plants, 10(4), 572-580.
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4

Hydrodynamic Plasmid Delivery in Mice

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Plasmids for hydrodynamic tail vein injection were prepared using the NucleoBond Xtra Maxi Plus EF Kit (Macherey-Nagel) according to the manufacturer’s instructions. Before injection, we diluted plasmid DNA in Ringer’s solution (0.9% NaCl, 0.03% KCl, 0.016% CaCl2) and a volume equivalent to 10% of mouse body weight was administered via the lateral tail vein in 5–8 s into 6–8-week-old mice [10 (link), 59 (link)]. The amount of plasmid DNA was 50 μg for each of the constructs mixed with 4 μg of the transposase helper plasmid.
Kopasz A.G., Pusztai D.Z., Karkas R., Hudoba L., Abdullah K.S., Imre G., Pankotai-Bodó G., Migh E., Nagy A., Kriston A., Germán P., Drubi A.B., Molnár A., Fekete I., Dani V.É., Ocsovszki I., Puskás L.G., Horváth P., Sükösd F, & Mátés L. (2022). A versatile transposon-based technology to generate loss- and gain-of-function phenotypes in the mouse liver. BMC Biology, 20, 74.
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