D glucose monohydrate

Manufactured by Thermo Fisher Scientific

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D(+)-glucose monohydrate is a chemical compound that is the monohydrate form of the simple sugar glucose. It is a white crystalline solid that is commonly used as a standard reference material and in various analytical and research applications in laboratories.

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D-glucose (488 citations)

4 protocols using d glucose monohydrate

Bacterial Cellulose Production from Winery Waste

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Komagateibacter xylinus as a pure freeze-dried culture was obtained from “Colección Española de Cultivos Tipo” (CECT) (Paterna, Spain). Grape bagasse from Vitis vinifera L. Garnacha Tintorera, which contains skins, pulp, seeds, and stems, was obtained after the pressing process in winemaking. The bagasse raw material was kindly provided by a local winery (42°33′58.2″ N 7°40′37.4″ W) in the Ribeira Sacra region (Lugo, Spain). Potato samples (Solanum tuberosum) were gently supplied by a local company called Pitita’s Farm (Dozón, Spain). They were harvested at the geocoordinates 42°36′14.0″ N 8°01′24.6″ W.
Yeast extract, methanol, ethanol, sulfuric acid (95–97%), and sodium carbonate were supplied by Scharlau Microbiology (Barcelona, Spain). Standards of gluconic acid, D(+)-glucose monohydrate (99%), D-xylose (99%), L(+)-arabinose (99%), 5-hydroxymethyl furfural (HMF) (98%) and furfural (99%) standards were purchased from Acros organics (Geel, Belgium). Analytical HPLC grade reagents and solvents were exclusively employed for the HPLC analysis. Folin–Ciocalteu reagents were supplied by Panreac (Barcelona, Spain).

Vázquez M., Puertas G, & Cazón P. (2023). Processing of Grape Bagasse and Potato Wastes for the Co-Production of Bacterial Cellulose and Gluconic Acid in an Airlift Bioreactor. Polymers, 15(19), 3944.

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Bacterial Cellulose Production from Winery Waste

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Detailed Characterization of Chelating Agents

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Unless otherwise stated, all reagents were used without further purification. Xylenol orange, meglumine, L-(+)-lactic acid, sodium acetate, oxidized (GSSG) and reduced (GSH) glutathione, GdCl₃ and DTPA were purchased from Sigma Aldrich. DOTA, and Gd-DOTA were purchased from Macrocyclics. All oligonucleotides were purchased from IDTDNA. Modified oligonucleotides (biotinylated, fluorescein and dabcyl tagged) are ordered purified via reverse-phase HPLC. D-glucose monohydrate, HEPES, Na₂HPO₄, NaH₂PO₄, NaHCO₃, NaCl, KCl, HCl, NaOH, MnCl₂.4H₂O, CaCl₂, NiCl₂.6H₂O were obtained from Fisher Chemical. FeCl₂.4H₂O, CuCl, CuCl₂.2H₂O, FeCl₃, MgCl₂, ZnCl₂, CrCl₃ from Acros Organics, CoCl₂.6H₂O from Spectrum Chemicals, Na₂CO₃.H₂O from JT Baker, and NaHSO₄.H₂O from Alfa Aesar. Magnevist® (Bayer) and Ablavar® (Lantheus Medical Imaging) were kindly donated by the University of California San Francisco Department of Radiology and Biomedical Imaging.

Edogun O., Nguyen N.H, & Halim M. (2016). Fluorescent single-stranded DNA-based assay for detecting unchelated Gadolinium(III) ions in aqueous solution. Analytical and bioanalytical chemistry, 408(15), 4121-4131.

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Optimization of Serratia rubidaea Growth

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First, 15 mL sterile conical tubes were filled with 60% of their volume with either MM supplemented with 30 g/L of NaCl (MM) [77 (link)] or MB. Each set of tubes was supplemented with 5 g/L of different carbon sources: sugars such as D-(+)-glucose monohydrate, sucrose (both from Fisher Scientific, Loughborough, UK), arabinose, fructose (both from Merck, Darmstadt, Germany), D-(+)-maltose monohydrate, mannose, D-(+)-melezitose monohydrate, D-(+)-raffinose pentahydrate, D-(+)-trehalose dihydrate, xylose (all from Sigma-Aldrich, Sigma-Aldrich, St. Louis, MO, USA) and mannitol (from Panreac Applichem, Barcelona, Spain); the amino acid glutamic acid (Sigma-Aldrich, St. Louis, MO, USA) and its sodium salt, sodium glutamate monohydrate (Merck, Darmstadt, Germany); the polysaccharides starch (Merck, Darmstadt, Germany) and inulin (Sigma-Aldrich, St. Louis, MO, USA); the polysorbate surfactant Tween 80 (Merck, Darmstadt, Germany); and glycerol (Panreac Applichem, Barcelona, Spain). Inoculation of each tube was performed with an isolated colony of Serratia rubidaea, taken from marine agar plates incubated for 24 h. The tubes were incubated horizontally at 30 °C and 150 rpm for 24 h. The assays were performed at least in duplicate.

Pereira R.F, & de Carvalho C.C. (2024). Improving Bioprocess Conditions for the Production of Prodigiosin Using a Marine Serratia rubidaea Strain. Marine Drugs, 22(4), 142.

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