The salinity tolerance of PASD solution was tested using NaCl, CaCl2, and MgCl2. The apparent viscosities of polyampholyte solutions in different saline concentrations were determined by HAAKE MARS 60 rotational rheometer (Thermo, Karlsruhe, Germany). The apparent viscosities were determined using the cone/plate geometry (diameter 35 mm, angle 1°, plate to plate gap 0.052 mm) under a shear rate of 7.34 s−1 at room temperature.
Haake mars 60 rotational rheometer
Manufactured by Thermo Fisher Scientific
Sourced in Germany, United States
The HAAKE MARS 60 is a rotational rheometer designed for rheological measurements. It provides precise control and measurement of shear stress, shear rate, and viscosity of various materials. The instrument is capable of performing a range of rheological tests, including flow curves, oscillation, and creep-recovery analyses.
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2 protocols using haake mars 60 rotational rheometer
1
Saline Tolerance and Viscosity of Polyampholyte
2
Characterization of JAM-A@NV Hydrogel
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The JAM-A@NV Gel was prepared by thoroughly stirring and mixing Poloxamer 407 hydrogel (Solarbio biotechnology, China) and PBS containing JAM-A@NVs (v/v, 20%), within an ice bath. In addition, a Calcein/PI cell viability/cytotoxicity assay kit (Beyotime biotechnology, China) was utilized to assess the potential toxicity of Poloxamer 407 hydrogel towards DPCs.
To facilitate the examination of the microstructure of the JAM-A@NV Gel, the hydrogel underwent a pre-freeze drying using a vacuum freeze dryer (SCIENTZ-10 N, SCIENTZ, China) for a duration of 36 h. Subsequently, the sample was acquired to examine its microstructure using a scanning electron microscope (SEM, ZEISS GeminiSEM 300, ZEISS, Germany). In addition, a Haake Mars60 rotational rheometer (Thermo Scientific, USA) was used to conduct the viscosity and rheological characterization studies. The experimental parameters include a temperature range spanning from 4 °C to 49 °C, with a temperature increment of 3 °C. The viscosity modulus was measured at a shear rate of 10 S−1, while the rheological modulus was determined at a frequency of 1 Hz. Additionally, the amplitude used for the measurements was set at 1%.
To facilitate the examination of the microstructure of the JAM-A@NV Gel, the hydrogel underwent a pre-freeze drying using a vacuum freeze dryer (SCIENTZ-10 N, SCIENTZ, China) for a duration of 36 h. Subsequently, the sample was acquired to examine its microstructure using a scanning electron microscope (SEM, ZEISS GeminiSEM 300, ZEISS, Germany). In addition, a Haake Mars60 rotational rheometer (Thermo Scientific, USA) was used to conduct the viscosity and rheological characterization studies. The experimental parameters include a temperature range spanning from 4 °C to 49 °C, with a temperature increment of 3 °C. The viscosity modulus was measured at a shear rate of 10 S−1, while the rheological modulus was determined at a frequency of 1 Hz. Additionally, the amplitude used for the measurements was set at 1%.
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