PEG (5 kDa, α-methoxy-ω-NHS ester activated) was purchased from NanoCS (New York, NY). Branched PEI (bPEI), deoxycholate (DOC), N-hydroxy succinimide, 4-dimethylaminopyridine, 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide hydrochloride (EDC HCl), palmitoyl chloride, resazurin sodium salt, sulforhodamine B based in vitro toxicology assay kit (TOX6), and poly(L-lysine) (PLL, 30-70 kDa) were purchased from Sigma Aldrich (St. Louis, MO). RNAiMAX, 4-12% NuPAGE Bis-Tris precast gels, HEPES buffer (pH 8.0, 1 M), MES buffer (0.1 M, pH 5.0), RIPA buffer, pyridine, NuSieve GTG agarose, dialysis membrane with molecular cut-off (MWCO) of 6-8 and 100 kDa, and other organic solvents were purchased from Fisher Scientific (Waltham, MA). siRNA (5′-GUUGGCACCAGCAGCGCACUU-3′) was purchased from GE Dharmacon (Lafayette, CO). Opti-MEM was purchased from Life Technologies (Carlsbad, CA). Mitochondrial ToxGlo™ assay kit and Bradford assay kit were purchased from Promega (Madison, WI). McCoy's 5A, 0.05% trypsin/EDTA, and phosphate buffered saline (PBS) were from GE Healthcare (Logan, UT). Fetal bovine serum (FBS) was purchased from Atlanta Biologicals (Flowery Branch, GA).
Nusieve gtg agarose
Manufactured by Thermo Fisher Scientific
NuSieve GTG agarose is a high-resolution agarose used for electrophoresis. It is designed for the separation of small DNA fragments and is suitable for DNA fragment analysis, PCR product visualization, and other DNA separation applications.
Automatically generated - may contain errors
Lab products found in correlation
4 dimethylaminopyridine, by Merck Group (1 mentions)
Ripa buffer, by Thermo Fisher Scientific (1 mentions)
Opti mem, by Thermo Fisher Scientific (1 mentions)
Rnaimax, by Thermo Fisher Scientific (1 mentions)
Mes buffer, by Thermo Fisher Scientific (1 mentions)
Phosphate buffered saline (pbs), by GE Healthcare (1 mentions)
Hepes buffer, by Thermo Fisher Scientific (1 mentions)
Pyridine, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Atlanta Biologicals (1 mentions)
Resazurin sodium salt, by Merck Group (1 mentions)
1 ethyl 3 3 dimethylaminopropyl carbodiimide hydrochloride edc hcl, by Merck Group (1 mentions)
Palmitoyl chloride, by Merck Group (1 mentions)
Mitochondrial toxglo assay kit, by Promega (1 mentions)
Mccoy s 5a, by GE Healthcare (1 mentions)
Sulforhodamine b based in vitro toxicology assay kit tox6, by Merck Group (1 mentions)
Neutral red, by Thermo Fisher Scientific (1 mentions)
Nusieve gtg agarose, by Lonza (1 mentions)
2 protocols using nusieve gtg agarose
1
Synthesis and Characterization of PEGylated Polyplexes
2
Murine Norovirus Titration Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Murine norovirus (MNV-1) virus stocks were prepared as described (Wobus et al., 2004) . The generation numbers used heretofore refer to number of passages since the start of the present experiment; the 0 th generation refers to the 6 th passage of MNV-1.CW3 (referred to as MNV-1 throughout) (Thackray et al., 2007) .
MNV-1 samples were titrated by 10-fold dilution plaque assays in duplicate as previously described (Gonzalez-Hernandez et al., 2012; Wobus et al., 2004) in Lab 1. Lab 2 employed a protocol with the following modifications. Two hundred microliters of MNV-1 samples, in tenfold dilutions using complete D-MEM with 2% FBS, were inoculated onto wells for titering. Plates were incubated for an hour at 37 o C (Lab 2) and plates were overlaid with 2 ml of D-MEM with 0.7% NuSieve GTG agarose (Lonza Inc., Allendale, NJ). Plates were incubated for 24 hours before a second overlay of 2 ml of D-MEM with 0.7% NuSieve GTG agarose and 0.07% neutral red (Fisher Scientific, Pittsburgh, PA) was added. Plaques were counted following an additional 48 hours of incubation.
MNV-1 samples were titrated by 10-fold dilution plaque assays in duplicate as previously described (Gonzalez-Hernandez et al., 2012; Wobus et al., 2004) in Lab 1. Lab 2 employed a protocol with the following modifications. Two hundred microliters of MNV-1 samples, in tenfold dilutions using complete D-MEM with 2% FBS, were inoculated onto wells for titering. Plates were incubated for an hour at 37 o C (Lab 2) and plates were overlaid with 2 ml of D-MEM with 0.7% NuSieve GTG agarose (Lonza Inc., Allendale, NJ). Plates were incubated for 24 hours before a second overlay of 2 ml of D-MEM with 0.7% NuSieve GTG agarose and 0.07% neutral red (Fisher Scientific, Pittsburgh, PA) was added. Plaques were counted following an additional 48 hours of incubation.
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