β-Galactosidase (β-Gal), horseradish peroxidase, alkaline phosphatase, papain, pepsin, and α-mannosidase were purchased from Beyotime (Shanghai, China). Lysozyme, glucose, bovine serum albumin, γ-globulin, transferrin, mannitol tryptophan and proline were purchased from Solarbio (Beijing, China). Ciprofloxacin, ampicillin, kanamycin, dimethyl sulfoxide (DMSO), Polypropylene pyrrolidone K30 (PVP-K30, MW = 40,000) and all other chemicals and solvents were purchased from Sigma-Aldrich (St. Louis, United States). Luria-Bertani broth (LB) medium was purchased from BD Biosciences (San Jose, CA). All aqueous solutions were prepared with Milli-Q water (≥18 MΩ, Milli-Q, Millipore).
Luria bertani broth lb medium
Manufactured by BD
Sourced in United States
Luria-Bertani broth (LB medium) is a nutrient-rich growth medium commonly used for the cultivation of bacteria, particularly Escherichia coli. It provides the necessary nutrients and a suitable environment for the proliferation of bacterial cells.
Automatically generated - may contain errors
Lab products found in correlation
Alkaline phosphatase, by Beyotime (1 mentions)
Transferrin, by Solarbio (1 mentions)
Lysozyme, by Solarbio (1 mentions)
Proline, by Solarbio (1 mentions)
Papain, by Beyotime (1 mentions)
Kanamycin, by Merck Group (1 mentions)
Milli q, by Merck Group (1 mentions)
Milli q water, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Ampicillin, by Merck Group (1 mentions)
Ciprofloxacin, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Solarbio (1 mentions)
2 protocols using luria bertani broth lb medium
1
Enzymatic Activity Assays Optimization
2
Bacterial Strain Culture and Antibiotic Selection
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The bacterial strains and plasmids used in this study are listed in Table 1. E. coli DH5α and BL21 cells were cultured in Luria-Bertani broth (LB) medium (BD Biosciences, Franklin Lakes, NJ, USA) containing the appropriate antibiotics (100 μg/ml ampicillin sodium salt or 50 μg/ml kanamycin sulfate). S. aureus and its derivative strains were cultured in tryptic soy broth (TSB) medium (BD Biosciences, Franklin Lakes, NJ, USA) containing erythromycin (2.5 μg/ml) or chloramphenicol (15 μg/ml) as necessary.
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