Antirat igg antibody

Manufactured by Merck Group

Sourced in United Kingdom

The Antirat IgG antibody is a laboratory reagent used for the detection and quantification of rat immunoglobulin G (IgG) in various biological samples. It is a specific antibody that binds to rat IgG, allowing for its identification and measurement through various immunoassay techniques.

Automatically generated - may contain errors

Lab products found in correlation

Anti vimentin antibody, by Merck Group (1 mentions) A9037, by Merck Group (1 mentions) Cl xposure film, by Thermo Fisher Scientific (1 mentions)

2 protocols using antirat igg antibody

Identifying Primary Atrial Mechanocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Identification of primary atrial mechanocytes was performed by vimentin immunostaining. First, mechanocytes were washed 2 to 3 times with PBS, fixed with 4% polyformaldehyde for 20 minutes, rinsed with PBS three times, treated with 0.5% Triton-100 X at room temperature for 20 min, and rinsed with PBS three times for 2 min each time. Cells were blocked with 1% bovine serum albumin overnight and then they were incubated with the antibody (anti-Vimentin antibody; Sigma, USA) at a 1:100 dilution at 4°C overnight. They were then rinsed with PBS three times for 2 min each time, and the next antibody was added at a 1:100 dilution (sheep anti-rabbit labelled with fluorescent dye Alexa Flour 488 and the antirat IgG antibody; Sigma). The cells were incubated with the fluorescent antibodies for 30 min at 37°C, and PBS was used to rinse the cells three times, 2 min each time; then, the cells were incubated with 1:1,000 Drap5 for 15 min to dye the nuclei, and laser scanning confocal microscopy was used to observe and photograph the cells.

Chen Y., Chang G., Chen X., Li Y., Li H., Cheng D., Tang Y, & Sang H. (2020). IL-6-miR-210 Suppresses Regulatory T Cell Function and Promotes Atrial Fibrosis by Targeting Foxp3. Molecules and Cells, 43(5), 438-447.

+ Open protocol

+ Expand

Detection of FSVS-tagged Dα6 Protein

Check if the same lab product or an alternative is used in the 5 most similar protocols

Alpha-Btx pull-down enriched or unenriched protein samples were treated with 1% DTT or left untreated, boiled at 60 °C for 8 min, separated by PAGE and transferred onto a nitrocellulose membrane (1704158, Trans-Blot Turbo Mini, Bio-Rad Laboratories, Inc, Watford, United Kingdom).
Ponceau S staining was used as a sample loading control. FSVS-tagged Dα6 (3xFLAG-StrepII-mVenus-StrepII) was detected with anti-GFP (Ab252881, Abcam, Cambridge, United Kingdom). 5% skimmed milk power dissolved in TBS-T was used for blocking and membranes were incubated for 16 hr at 4 °C with the α-GFP antibody (1:1000 concentrated in blocking solution) followed by anti-rat IgG antibody for 1 hour (A9037, Sigma-Aldrich, Haverhill, United Kingdom). Immunoblots were treated with an ECL chemiluminescent detection solution (45-000-999, GE Healthcare, Chalfont St. Giles, United Kingdom) exposed for 10 s to CL-XPosure films (10465145, Thermo Scientific, Bishop’s Stortford, United Kingdom) and visualised using an x-ray developer (1170-1-8000, Protec GmbH, Oberstenfeld, Germany). Two biological replicates were performed.

Korona D., Dirnberger B., Giachello C.N., Queiroz R.M., Popovic R., Müller K.H., Minde D.P., Deery M.J., Johnson G., Firth L.C., Earley F.G., Russell S, & Lilley K.S. (2022). Drosophila nicotinic acetylcholine receptor subunits and their native interactions with insecticidal peptide toxins. eLife, 11, e74322.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!