Cells that were grown on coverslips under the indicated conditions were fixed in 4% formaldehyde at 20 °C for 15 min and permeabilized in methanol at –20 °C for 10 min. The coverslips were then incubated at 20 °C for 3 h with anti-HCV core protein polyclonal (Abcam, Cat No. ab58713, 1:200) and anti-HBx monoclonal (Santa Cruz, Cat No. sc-57760, 1:500 dilution) antibodies. The cells were then incubated with anti-rabbit IgG-rhodamine (Invitrogen, Cat No. 31670, 1:200 dilution) and anti-mouse IgG-FITC (Sigma-Aldrich, Cat No. F0257-1ML, 1:100 dilution) antibodies at 20 °C for 1 h. The slides were prepared with UltraCruz mounting medium (Santa Cruz Biotechnology, Cat No. sc-24941) were visualized using an Eclipse fluorescence microscope (Nikon).
Anti hbx monoclonal
Manufactured by Santa Cruz Biotechnology
Anti-HBx monoclonal is a laboratory research tool used to detect and study the HBx protein, which is a regulatory protein expressed by the hepatitis B virus. This monoclonal antibody can be used in various immunoassay techniques, such as Western blotting and immunohistochemistry, to identify and analyze the HBx protein in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Ultracruz mounting medium, by Santa Cruz Biotechnology (2 mentions)
Eclipse fluorescence microscope, by Nikon (2 mentions)
Anti rabbit igg rhodamine, by Thermo Fisher Scientific (2 mentions)
Anti mouse igg fitc, by Merck Group (1 mentions)
Anti mouse igg fluorescein isothiocyanate fitc, by Merck Group (1 mentions)
2 protocols using anti hbx monoclonal
1
Immunofluorescence Imaging of HCV Core and HBx
2
Immunofluorescence Assay for HCV and HBx
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells that were grown on coverslips under the indicated conditions were fixed in 4% formaldehyde at 20°C for 15 min and permeabilized in methanol at −20°C for 10 min. The coverslips were then incubated at 20°C for 3 h with anti-HCV core protein polyclonal (catalog no. ab58713; Abcam) (1:200) and anti-HBx monoclonal (catalog no. sc-57760; Santa Cruz) (1:500) antibodies (Fig. 1C and D ; see also Fig. S1D at https://dv.pusan.ac.kr/SynapDocViewServer/viewer/doc.html?key=402882e382e741b101845c07289e5ac8&convType=html&convLocale=ko&contextPath=/SynapDocViewServer/ ) and with anti-HA polyclonal (catalog no. ab9110; Abcam) (1:200) and anti-HCV core protein monoclonal (catalog no. ab-2740; Abcam) (1:200) antibodies (Fig. 2D ). The cells were then incubated with anti-rabbit IgG–rhodamine (catalog no. 31670; Invitrogen) (1:200 dilution) and anti-mouse IgG–fluorescein isothiocyanate (FITC) (catalog no. F0257-1ML; Sigma-Aldrich) (1:100 dilution) antibodies at 20°C for 1 h. The slides were prepared with UltraCruz mounting medium (catalog no. sc-24941; Santa Cruz Biotechnology) and visualized using an Eclipse fluorescence microscope (Nikon).
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