Goat anti rabbit igg h l hrp antibody

The Q808 compound was a kind gift from the Academy of Chinese Medical Sciences of Jilin Province, and the synthesis conformation of Q808 has been verified (Zhang et al., 2009 (link)). The other drugs and reagents, such as PTZ, Tween-80, and CMC-Na, were purchased from Sigma-Aldrich Chemical Company (St. Louis, United States). ACE, β-tubulin, and Goat Anti-Rabbit IgG (H + L) HRP antibody were purchased from Affinity Biosciences (Affinity, United States).

The knee joints were fixed in 4% paraformaldehyde and decalcified with 20% ethylene diamine tetraacetic acid (EDTA). The tissues were embedded in paraffin and cut into sections. The SOFA and H&E staining were performed using Safranin O/Fast green Kit (Solarbio, China) or Hematoxylin-Eosin Staining Kit (Beyotime, China). The Osteoarthritis Research Society Internationa (OARSI) score was used to analyze the severity of cartilage degeneration (29 (link)). For IHC staining, the sections were dewaxed and treated with Antigen Retrieval Solution (Beyotime, China). The sections were incubated successfully with blocking buffer (Beyotime, China), antibodies, and diaminobenzidine (DAB) staining solution (Beyotime, China). The antibodies used in IHC staining included anti-MMP-13 (1:200; Affinity, China), anti-Collagen II (1:100; Invitrogen, USA), anti-GPX4 (1:100; Affinity, China), anti-ACSL4 (1:200; Affinity, China), anti-SLC7A11 (1:100; Affinity, China), and Goat Anti-Rabbit IgG (H+L) HRP antibody (1:500; Affinity, China).