C33A cells were fixed with 1% formaldehyde for 10 min at room temperature and chromatin extracted as described in Ryme et al. (2009 (link)). For RNA regulatory protein 1.5–2% formaldehyde was used (Görnemann et al. 2005 (link)). The chromatin was fragmented by sonication to fragments with a mean length of 500 bp. The antibodies used: BRM, SAM68, hnRNPL, hnRNPU, BAF155/SMARCC1, BAF250/ARID1, BAF200/ARID2, and BAF180/PBRM1 were purchased from Abcam, BAF200/ARID2 and BAF180/PBRM1 were from Bethyl Laboratories Inc and BRD9 were from Cell signalling and Anova (Supplementary Table S6). Primers used in the analysis are presented in Supplementary Table S5. The standard was SD, and p value calculated according to Student’s t test.
Gañez-Zapater A., Mackowiak S.D., Guo Y., Tarbier M., Jordán-Pla A., Friedländer M.R., Visa N, & Östlund Farrants A.K. (2022). The SWI/SNF subunit BRG1 affects alternative splicing by changing RNA binding factor interactions with nascent RNA. Molecular Genetics and Genomics, 297(2), 463-484.
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