Ifn betamouse procartaplex simplex kit

Manufactured by Thermo Fisher Scientific

The IFN betaMouse ProcartaPlex Simplex Kit is a laboratory assay kit designed to measure the concentration of interferon-beta (IFN-beta) in mouse samples. The kit utilizes a multiplex bead-based immunoassay technology to quantify the target analyte.

Automatically generated - may contain errors

Lab products found in correlation

Bio plex pro mouse cytokine 23 plex, by Bio-Rad (2 mentions) Il 28b ifn lambda 3 mouse elisa kit, by Thermo Fisher Scientific (2 mentions) Bio plex pro tgf β assay, by Bio-Rad (2 mentions) T per, by Thermo Fisher Scientific (2 mentions) Bio plex 200, by Bio-Rad (2 mentions) Protease inhibitor cocktail, by Roche (2 mentions)

Close spelling variants of this product

ProcartaPlex™ Simplex Kit ProcartaPlex kit

2 protocols using ifn betamouse procartaplex simplex kit

Cytokine Profiling of Lung Tissue after Vaccine Adjuvants

Check if the same lab product or an alternative is used in the 5 most similar protocols

At 24 and 48 hours after vaccination with OVA and various adjuvants,
lungs were harvested for cytokine analysis Five hundred microliter of the
mammalian tissue protein extraction reagent (T-PER) (Thermo Scientific)
containing protease inhibitor cocktails (Roche, Indianapolis, IN), was added
to the lung tissue and homogenized in a tissue homogenizer. Subsequently,
tissue lystate was centrifuged at 10,000 × g for 5 minutes at 4C.
Supernatants were collected and protein concentrations in extracts were
quantified using BCA method and 1mg of total lysate was added for each well
in a 96-well plate. Cytokines were quantified using Bio-Plex Pro Mouse
Cytokine 23-plex and Bio-Plex Pro TGF-β Assays (Bio-Rad), IFN beta
Mouse ProcartaPlex Simplex Kit and IL-28B/(IFN lambda 3) Mouse ELISA kit
(eBioscience), as recommended by the manufacturer. The samples were acquired
and analyzed using Bioplex-200 with the Bioplex Manager 6.1.1. The data were
normalized by (the amount of cytokine/ml) * (extraction volume) divided by
the weight of the lung tissue (mg).

Marinaik C.B., Kingstad-Bakke B., Lee W., Hatta M., Sonsalla M., Larsen A., Neldner B., Gasper D.J., Kedl R.M., Kawaoka Y, & Suresh M. (2020). Programming Multifaceted Pulmonary T Cell Immunity by Combination Adjuvants. Cell Reports Medicine, 1(6), 100095.

+ Open protocol

+ Expand

Cytokine Analysis in Lung Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

At 24 and 48 hours after vaccination with OVA and various adjuvants, lungs were harvested for cytokine analysis Five hundred microliter of the mammalian tissue protein extraction reagent (T-PER) (Thermo Scientific) containing protease inhibitor cocktails (Roche, Indianapolis, IN), was added to the lung tissue and homogenized in a tissue homogenizer. Subsequently, tissue lystate was centrifuged at 10,000 x g for 5 minutes at 4C. Supernatants were collected and protein concentrations in extracts were quantified using BCA method and 1mg of total lysate was added for each well in a 96-well plate. Cytokines were quantified using Bio-Plex Pro Mouse Cytokine 23-plex and Bio-Plex Pro TGF-β Assays (Bio-Rad), IFN beta Mouse ProcartaPlex Simplex Kit and IL-28B/(IFN lambda 3) Mouse ELISA kit (eBioscience), as recommended by the manufacturer. The samples were acquired and analyzed using Bioplex-200 with the Bioplex Manager 6.1.1. The data were normalized by (the amount of cytokine/ml) ∗ (extraction volume) divided by the weight of the lung tissue (mg).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!