Raw milk was collected and stored at 20 °C before use. The volume was divided into aliquots of 20 mL, which were inoculated with 1000, 100, and 10 spores. A negative control was also added consisting of milk without spores. Each sample was treated with 20 mL of Buffer F (EDTA 100 mM (Sigma-Aldrich, St. Louis, MO, USA), sodium citrate 250 mM (Carlo Erba, Cornaredo, Italy), Tris HCl 200 mM (Sigma-Aldrich, St. Louis, MO, USA), Triton X-100 0.01%; final pH 7.5 (Sigma-Aldrich, St. Louis, MO, USA)) and incubated in a water bath at 65 °C for 1 h. After that, samples were vortexed for 10 s and centrifuged at 13,000 rpm for 30 min at 40 °C. The supernatant was discarded, paying attention to avoid the mixing of pellets with the residual fat. The experiment was performed in triplicates. Moreover, an aliquot of each sample was analyzed to check the right number of recovered spores, through the plating of decimal dilutions on RCM agar (Oxoid, Altrincham, UK).
Sodium citrate
Manufactured by Carlo Erba
Sourced in United States, Italy
Sodium citrate is a chemical compound that acts as an anticoagulant. It is commonly used in laboratory settings to prevent blood samples from clotting.
Automatically generated - may contain errors
2 protocols using sodium citrate
1
Milk Spore Extraction and Enumeration
2
Silver Nanoparticle Dispersion Characterization
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Nearly monodisperse sodium citrate stabilized silver AgNPs dispersions of 20, 30, 40, 60, 70 and 100 nm at a nominal concentration of 20 mgL -1 were kindly donated by the Joint Research Centre, Institute for Health and Consumer Protection, Ispra (Italy). To avoid silver particle degradation or precipitation, dispersions were stored at 4 °C and protected from prolonged exposure to light.
Information regarding the sizes and the actual total silver concentrations, determined with complementary techniques by the JRC are reported in Tables 1 and2 respectively.
NaOH and sodium citrate (Carlo Erba Reagents -Italy) were used to prepare the mobile phase (eluent) for the SdFFF instrument.
All solutions were prepared using ultrapure deionised water (18 MΩ cm -1 ) obtained from a MilliQ system (Merck Millipore Milan, Italy).
Information regarding the sizes and the actual total silver concentrations, determined with complementary techniques by the JRC are reported in Tables 1 and2 respectively.
NaOH and sodium citrate (Carlo Erba Reagents -Italy) were used to prepare the mobile phase (eluent) for the SdFFF instrument.
All solutions were prepared using ultrapure deionised water (18 MΩ cm -1 ) obtained from a MilliQ system (Merck Millipore Milan, Italy).
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