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Epoch microplate spectrophotometer

Manufactured by Omega Bio-Tek
Sourced in United States

The Epoch Microplate Spectrophotometer is a laboratory instrument used to measure the absorbance of light in microplate samples. It is capable of detecting and quantifying various biomolecules, such as proteins, nucleic acids, and other substances, by analyzing their optical properties.

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2 protocols using epoch microplate spectrophotometer

1

Cell Proliferation Assay with CCK8

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Cells exhibiting logarithmic growth were seeded into 96-well plates at a density of 5 × 104 cells/well and incubated overnight. Cell proliferation was determined with a CCK8 kit (E606335, Shanghai Sangon Biotechnology Co. Ltd., Shanghai, China). After 0, 24, 48, and 72 h of cell culture, 10 μL of CCK8 reagent was added to each well, respectively. After incubation for an hour in a humidified incubator at 37°C, the optical density (OD) was measured at a wavelength of 450 nm with an Epoch Microplate Spectrophotometer (Omega Bio-Tek, Norcross, GA, USA). Three replicate wells were set for each group.
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2

Cell Viability Assay for Chemotherapeutic Screening

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Cells were seeded into 96-well plates with 1 × 104 cells per well and cultured for 24 h. When IC50 was detected, OC cells were incubated with 0, 5, 10, 20, 40 and 80 μmol/L CP (Sigma-Aldrich) for 48 h. CP was soluble in water, and the mother solution was thus prepared with distilled water before the experiment and cryopreserved at—20℃ for subsequent cell viability test. The concentration of CP was 25 μg/mL and the cells were incubated with CP for 48 h. Each well was subsequently supplemented with 10 µL of CCK-8 solution (Sigma-Aldrich) for 1-h incubation in a 37℃ incubator for 1 h. The OD value of each well was measured using an Epoch microplate spectrophotometer (Omega Bio-tek Inc, Norcross, GA) at 450 nm.
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