Epoch microplate spectrophotometer

Cells exhibiting logarithmic growth were seeded into 96-well plates at a density of 5 × 10⁴ cells/well and incubated overnight. Cell proliferation was determined with a CCK8 kit (E606335, Shanghai Sangon Biotechnology Co. Ltd., Shanghai, China). After 0, 24, 48, and 72 h of cell culture, 10 μL of CCK8 reagent was added to each well, respectively. After incubation for an hour in a humidified incubator at 37°C, the optical density (OD) was measured at a wavelength of 450 nm with an Epoch Microplate Spectrophotometer (Omega Bio-Tek, Norcross, GA, USA). Three replicate wells were set for each group.

Cells were seeded into 96-well plates with 1 × 10⁴ cells per well and cultured for 24 h. When IC50 was detected, OC cells were incubated with 0, 5, 10, 20, 40 and 80 μmol/L CP (Sigma-Aldrich) for 48 h. CP was soluble in water, and the mother solution was thus prepared with distilled water before the experiment and cryopreserved at—20℃ for subsequent cell viability test. The concentration of CP was 25 μg/mL and the cells were incubated with CP for 48 h. Each well was subsequently supplemented with 10 µL of CCK-8 solution (Sigma-Aldrich) for 1-h incubation in a 37℃ incubator for 1 h. The OD value of each well was measured using an Epoch microplate spectrophotometer (Omega Bio-tek Inc, Norcross, GA) at 450 nm.