Thermo protein a g dynabeads

Manufactured by Thermo Fisher Scientific

Thermo Protein A/G Dynabeads are magnetic beads coated with Protein A and Protein G. These beads can be used to capture and purify antibodies from complex samples, such as cell culture supernatants or serum.

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Lab products found in correlation

Cocktail of protease and phosphatase inhibitors, by Merck Group (1 mentions) Chemidoc touch imaging system, by Bio-Rad (1 mentions) M per, by Thermo Fisher Scientific (1 mentions) Bca protein assay kit, by Thermo Fisher Scientific (1 mentions) Ge amersham imager 600, by GE Healthcare (1 mentions) Amersham imager, by Cytiva (1 mentions)

Spelling variants of this product

Dynabeads (2112 citations) Dynabeads Protein G (1538 citations) Dynabeads Protein A (819 citations) G Dynabeads (52 citations) Protein A/G (47 citations)

2 protocols using thermo protein a g dynabeads

GTPase Signaling Pathway Activation

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Cells were pelleted and lysed with M-PER (Thermo Scientific Pierce) supplemented with a cocktail of protease and phosphatase inhibitors (Sigma-Aldrich). Protein lysates were collected by centrifugation at 13,000 RPM for 10 min at 4°C and pre-cleared by incubation with Thermo Protein A/G Dynabeads (Thermo Scientific Pierce) for 3 hours at 4°C on a rotator. Pre-cleared protein lysates were quantified with the BCA Protein Assay Kit (Thermo Scientific Pierce), and 2 mg/ml lysates were prepared. We used anti-Gα_12/13, anti-LARG and anti-RhoA antibodies for pull-down and immunoblotting at the recommended dilutions. Cell lysates were separated by SDS-PAGE and transferred onto nitrocellulose membranes. Blots were scanned digitally using the ChemiDoc™ touch imaging system (Bio-Rad).

Feng F., Han H., Wu S, & Wang H. (2021). Crosstalk Between Abnormal TSHR Signaling Activation and PTEN/PI3K in the Dedifferentiation of Thyroid Cancer Cells. Frontiers in Oncology, 11, 718578.

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Protein interaction analysis by pull-down

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Whole cell lysates were prepared by using M-PER as mentioned above, and were pre-cleared by incubation with Thermo Protein A/G Dynabeads (Thermo Scientific Pierce) for 3 hours at 4°C on a rotator. Pre-cleared protein lysates were quantified with the BCA Protein Assay Kit, and 2 mg/ml lysates were prepared. We used anti-NIS, anti-LARG and anti-RhoA antibodies for pull-down and immunoblotting at the recommended dilutions. Cell lysates were separated by SDS-PAGE and transferred onto nitrocellulose membranes. Blots were scanned digitally using the GE Amersham Imager 600 (GE Healthcare Life Science).

Feng F., Yehia L., Ni Y., Chang Y.S., Jhiang S.M, & Eng C. (2018). A non-pump function of sodium iodide symporter in thyroid cancer via crosstalk with PTEN signaling. Cancer research, 78(21), 6121-6133.

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