1 2 dipalmitoyl phosphatidylcholine

Manufactured by Avanti Polar Lipids

Sourced in United States

1,2-dipalmitoyl-phosphatidylcholine is a synthetic phospholipid commonly used in laboratory research. It is a major component of biological cell membranes and serves as a model system for studying membrane structure and function.

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Lab products found in correlation

1 palmitoyl 2 oleoyl sn glycero 3 phosphatidylcholine, by Avanti Polar Lipids (2 mentions) Zearalenone, by Merck Group (1 mentions) Sodium selenate, by Merck Group (1 mentions) 1 2 dipalmitoyl 3 trimethylammonium propane, by Avanti Polar Lipids (1 mentions) Chloroform, by Merck Group (1 mentions) 1 1 dioctadecyl 3 3 3 3 tetramethylindodicarbocyanine perchlorate did, by Thermo Fisher Scientific (1 mentions) Alexa 488, by Thermo Fisher Scientific (1 mentions) 1 palmitoyl 2 oleoylphosphatidylcholine, by Avanti Polar Lipids (1 mentions) 1 2 dimyristoyl phosphatidylcholine, by Avanti Polar Lipids (1 mentions)

Close spelling variants of this product

Phosphatidylcholine

5 protocols using 1 2 dipalmitoyl phosphatidylcholine

Lipid Synthesis and Characterization

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1-Palmitoyl-2-oleoyl-sn-glycero-3- phosphatidylcholine (POPC) and 1,2-dipalmitoyl-phosphatidylcholine (DPPC) were obtained from Avanti Polar Lipids (Alabaster AL). N-palmitoyl sphingomyelin (16:0 SM) and N-oleoyl sphingomyelin (18:1^Δ9(c) SM) were produced by reacylation of lyso-SM with the desired fatty acyl residue and purified as described previously.^{13 and refs.} 1-Palmitoyl-2-(4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene-8-heptanoyl)-sn-glycero-3-phosphocholine (Me₄-BODIPY-8-PC or B-PC) was synthesized and purified as described earlier¹⁷ (Figure 1A).

Zhai X., Boldyrev I.A., Mizuno N.K., Momsen M.M., Molotkovsky J.G., Brockman H.L, & Brown R.E. (2014). Nanoscale Packing Differences in Sphingomyelin and Phosphatidylcholine Revealed by BODIPY Fluorescence in Monolayers: Physiological Implications. Langmuir, 30(11), 3154-3164.

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Lipid Preparation and Characterization

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1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine
(POPC) and 1,2-dipalmitoylphosphatidylcholine (DPPC) were obtained
from Avanti Polar Lipids (Alabaster, AL). N-Palmitoylsphingomyelin
(16:0 SM) and N-oleoylsphingomyelin (18:1^Δ9^(c) SM) were produced by reacylation of lyso-SM with the
desired fatty acyl residue and purified as described previously (ref (13 (link)) and references therein).
1-Palmitoyl-2-(4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene-8-heptanoyl)-sn-glycero-3-phosphocholine
(Me₄-BODIPY-8-PC or B-PC) was synthesized and purified
as described earlier¹⁷ (Figure 1A).

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Lipid Monolayer Characterization

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Zearalenone and sodium selenate were purchased from Sigma-Aldrich Company (St, Louis, MO). 1,2-dipalmitoyl-phosphatidylcholine (DPPC) and 1,2-dipalmitoyl-3-trimethylammonium-propane (DPTAP) were obtained from Avanti Polar Lipids (Alabaster, AL). Chloroform (Merck, Germany) was the spreading solvent. The subphase was re-distilled water, purified by a Milli-Q system, with a specific resistance above 18.2 MQ cm⁻¹.

Gzyl-Malcher B., Filek M., Rudolphi-Skórska E, & Sieprawska A. (2017). Studies of Lipid Monolayers Prepared from Native and Model Plant Membranes in Their Interaction with Zearalenone and Its Mixture with Selenium Ions. The Journal of Membrane Biology, 250(3), 273-284.

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Lipid and Phytosterol Composition Analysis

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1,2-dipalmitoylphosphatidylcholine (DPPC), monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG) were purchased from Avanti Polar Lipids (see Table S1 in the supplementary material for the fatty acid repartition). Canola phytosterols were a gift from Cognis France (Estarac, France) obtained from desodorization distillates of canola oil. Typical molar composition (calculated from usual normalized GC procedures) was: β-sitosterol (50 mol%), campesterol (40 mol%) and brassicasterol (10 mol%).

Kergomard J., Carrière F., Paboeuf G., Artzner F., Barouh N., Bourlieu C, & Vié V. (2022). Interfacial organization and phase behavior of mixed galactolipid-DPPC-phytosterol assemblies at the air-water interface and in hydrated mesophases. Colloids and surfaces. B, Biointerfaces, 217.

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Purification and Lipid Preparation for Biophysical Studies

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St I was purified from the sea anemone S. helianthus as previously described [1 (link)]. The lipids: 1-palmitoyl-2-oleoyl phosphatidylcholine (POPC); egg sphingomyelin (eggSM); 1,2-dimyristoyl phosphatidylcholine (DMPC); 1,2-dipalmitoyl phosphatidylcholine (DPPC); 1,2-distearoyl phosphatidylcholine (DSPC); 1,2-diarachidoyl phosphatidylcholine (DAPC); 1,2-dioleoyl phosphatidylcholine (DOPC) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). The fluorescent probes carboxyfluorescein (CF), 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine perchlorate (DiD), and Alexa 488, Alexa 555 C5 maleimide, Dextran 3000-Alexa 488 and Dextran 10000-Alexa 647 were obtained from Invitrogen (Eugene, OR, USA). Solvents and chemicals were of the highest commercial purity available.

Pedrera L., Ros U., Fanani M.L., Lanio M.E., Epand R.M., García-Sáez A.J, & Álvarez C. (2023). The Important Role of Membrane Fluidity on the Lytic Mechanism of the α-Pore-Forming Toxin Sticholysin I. Toxins, 15(1), 80.

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