5 thio 2 nitrobenzoic acid

The chemicals utilized during study: ammonium sulphate, 2,4-dinitro-phenyl-hydrazine, 1-chloro-2,4-dinitrobenzene, ethylenediaminetetraacetic acid, 5,5-di-thio-bi(2-nitrobenzoic acid), N-ethylmaleimide, nitro blue tetrazolium, reduced nicotinamide adenine dinucleotide, potassium dihydrogen phosphate, phenazinemethosulphate, sodium pyrophosphate, reduced glutathione, sodium azide, thiobarbituric acid, 5-thio-2-nitrobenzoic acid and trichloro acetic acid were procured from Sisco Research Laboratory, India. Bovine serum albumin, Bradford reagent and Pb-acetate were purchased from Sigma-Aldrich, St. Louis, USA. All primary antibodies (produced in rabbit) viz. anti-Bcl-2 (dilution 1:1000), anti-Bad (dilution 1:3000), anti-Cyt C (dilution 1:1000), anti-Apaf-1 (dilution 1:1000), anti-caspase 3 (dilution 1:1000), anti-caspase 8 (dilution 1:1000), anti-caspase 9 (dilution 1:1000), anti-Fas (dilution 1:2000), anti-Bid (dilution 1:1000) and anti-actin (dilution 1:3000) for immunoblotting were purchased from Sigma-Aldrich Chemical Company St. Louis, USA. Appropriate HRP conjugated secondary antibody (dilution 1:3000) produced in goat was also purchased from Sigma-Aldrich Chemical Company St. Louis, USA. Methanol, formic acid, acetic acid and acetonitrile were obtained from Merck, India.

Bovine serum albumin (BSA), Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), Bradford reagent, Collagenase type I, antibodies for immunoblotting were purchased from Sigma-Aldrich Chemical Company (St. Louis, MO), USA. Solvents (HPLC grade) were obtained from Merck, Mumbai, India. Kits for measurement of serum biochemical parameters were purchased from Span diagnostic Ltd., India. 1-Chloro-2,4-dinitrobenzene, ammonium sulphate, sodium arsenite (NaAsO₂), 2,4-dinitrophenylhydrazine, ethylene diaminetetraacetic acid, 5,5-dithiobis(2-nitrobenzoic acid), N-ethylmaleimide, nitro blue tetrazolium, reduced nicotinamide adenine dinucleotide, potassium dihydrogen phosphate, phenazinemethosulphate, sodium pyrophosphate, reduced glutathione, sodium azide, thiobarbituric acid, 5-thio-2-nitrobenzoic acid and trichloro acetic acid were purchased from Sisco Research Laboratory, Mumbai, India.

CA, cadmium chloride (CdCl₂), fetal bovine serum (FBS), Bradford reagent, bovine serum albumin (BSA), and cell culture media were obtained from Sigma-Aldrich Chemical Company, St. Louis, USA. Antibodies for Western blotting were obtained from Cell Signaling Technology, Beverley, USA, Denver, USA, and Bio-Rad, Hercules, USA. (NH₄)₂SO₄, 2,4-dinitrophenylhydrazine, CCl₃COOH, 5-thio-2-nitrobenzoic acid, 5,5-dithiobis(2-nitrobenzoic acid), 1-chloro-2,4-dinitrobenzene, ethylene diaminetetraacetic acid (EDTA), Na₄P₂O₇, NaN₃, KH₂PO₄, N-ethylmaleimide, nitro blue tetrazolium (NBT), phenazinemethosulphate, reduced glutathione (GSH), reduced nicotinamide adenine dinucleotide (NADH), and thiobarbituric acid (TBA) were bought from Sisco Research Laboratory, Mumbai, India. Solvents were bought from Merck, Mumbai, India. Kits for measurement of different biochemical parameters were purchased from Span diagnostic Ltd., Kolkata, India. ELISA kits were obtained from R&D Systems, Minneapolis, USA and Thermo Fisher Scientific Co., Waltham, USA.

CA, bovine serum albumin (BSA), Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), Bradford reagent, and collagenase type I were procured from Sigma-Aldrich Chemical Company, MO, USA. Antibodies for immunoblotting were bought from Novus Biologicals, CO, USA. The solvents (HPLC grade) were obtained from Merck, Mumbai, India. Kits for the measurement of different biochemical parameters were procured from Span Diagnostics Ltd., India. 1-Chloro-2,4-dinitrobenzene, (NH₄)₂SO₄, NaAsO₂, 2,4-dinitrophenylhydrazine, ethylenediaminetetraacetic acid, 5,5-dithiobis(2-nitrobenzoic acid), N-Ethylmaleimide, nitro blue tetrazolium, NADH, KH₂PO₄, phenazine methosulphate, Na₄P₂O₇, GSH, NaN₃, thiobarbituric acid, 5-thio-2-nitrobenzoic acid, and CCl₃COOH were obtained from Sisco Research Laboratory, Mumbai, India.

Cadmium chloride (CdCl₂), fetal bovine serum (FBS), Bradford reagent, APO transferrin, hydrocortisone, antibiotic antimycotic solution, and bovine serum albumin (BSA) were obtained from Sigma-Aldrich Chemical Company, St. Louis, USA. Dulbecco’s modified Eagle’s/Ham’s F12 (DMEM-F12) and Hank’s balanced salt solution (HBSS) were procured from Thermo Fisher Scientific, USA. Antibodies for immunoblotting were obtained from Cell Signaling Technology, Beverley, USA, Denver, USA, and Bio-Rad, Hercules, USA. Furthermore, 5,5-dithiobis(2-nitrobenzoic acid), 1-chloro-2,4-dinitrobenzene, (NH₄)₂SO₄, 2,4-dinitrophenylhydrazine, 5-thio-2-nitrobenzoic acid, CCl₃COOH, ethylene diaminetetraacetic acid, Na₄P₂O₇, NaN₃, KH₂PO₄, N-ethylmaleimide, nitro blue tetrazolium, phenazinemethosufhate, reduced glutathione, reduced nicotinamide adenine dinucleotide (NADH), and thiobarbituric acid were purchased from Sisco Research Laboratory, Mumbai, India. HPLC-grade solvents were brought from Merck, Mumbai, India. Kits for measurement of different biochemical parameters were purchased from Span diagnostic Ltd., India.