Epitope retrieval and immunohistochemical (IHC) staining were performed as previously described.26 (link) A polyclonal rabbit antibody against full-length human GSTA4 with cross-reactivity to murine Gsta4 (Abnova, Walnut, CA, USA; H00002941-D01P, 1:100) and anti-4-HNE serum (Alpha Diagnostic International Inc, San Antonio, TX, USA; HNE11-S, 1:1,000) were used as primary antibodies. Goat anti-rabbit IgG-HRP conjugate (Life Technologies, Grand Island, NY, USA) was used as secondary antibody. Chromogenic color development was performed using 3,3′-diaminobenzidine enhanced liquid substrate (Sigma) and nuclei counterstained by Mayer’s hematoxylin (Sigma). IHC staining for GSTA4 in human tissues was scored using a combination of stain intensity (0, negative; 1, weak; 2, moderate; 3, strong) and overall number of positive cells per 20X field (0, none; 1, < 10%; 2, 10 – 50%; 3, >50% positive cells).
Anti 4 hne serum
Manufactured by Alpha Diagnostic
Sourced in United States
The Anti-4-HNE serum is a laboratory reagent used for the detection and quantification of 4-hydroxynonenal (4-HNE), a biomarker of oxidative stress. It is a polyclonal antibody that specifically binds to 4-HNE adducts, allowing for the measurement of 4-HNE levels in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Mayer s hematoxylin, by Merck Group (1 mentions)
Goat anti rabbit igg hrp conjugate, by Thermo Fisher Scientific (1 mentions)
3 3 diaminobenzidine enhanced liquid substrate, by Merck Group (1 mentions)
Alexa fluor 594 conjugated goat anti rabbit igg secondary antibody, by Thermo Fisher Scientific (1 mentions)
4 hne, by Alpha Diagnostic (1 mentions)
2 protocols using anti 4 hne serum
1
Immunohistochemical Analysis of GSTA4
2
Measuring Hippocampal Oxidative Stress
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To evaluate level of oxidative stress in the hippocampus, we conducted immunofluorescence staining with paraformaldehyde-fixed brain tissue. Oxidative stress was detected by measuring the presence of the lipid peroxidation product, 4HNE (4-hydroxy-2-nonenal). Immunohistochemistry with 4HNE (Alpha Diagnostic Intl. Inc., San Antonio, TX, USA) antibodies was conducted according as previously described manual [64 (link)]. Brain sections were immersed in a polyclonal rabbit anti-4HNE serum (diluted 1:500, Alpha Diagnostic Intl. Inc., San Antonio, TX, USA) with the PBS containing 0.3% TritonX-100 for overnight in a 4 °C incubator. After we washed the sections three times for 10 min with PBS, these sections were also immersed in a solution of Alexa Fluor 594-conjugated goat anti-rabbit IgG secondary antibody (diluted 1:250, Invitrogen, Grand Island, NY, USA) for two hours at RT. The sections were laid on gelatin-coated slides in order to observe under a microscope. To measure the oxidative injury, we used Image J (v. 1.47c.) program and measured the mean gray value [63 (link)].
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!