Conditioned media were collected at day 7 and levels of secreted bone morphogenetic protein-2 (BMP-2; Elabscience, Houston, TX, USA), bone morphogenetic protein-4 (BMP-4; Elabscience, Houston, TX, USA), vascular endothelial growth factor A (VEGF-A; Elabscience, Houston, TX, USA), and fibroblast growth factor-2 (FGF-2; Elabscience, Houston, TX, USA) were measured by immunoassay. For each of these proteins, a titration curve was designed with provided standards. Equal volumes (100 μL) of the collected culture media were immobilised on antibody-coated wells and immunoassays of the investigated proteins were developed according to the manufacturer’s instructions. Absorbance of the developed reactions was measured by a microplate reader (BioRad Laboratories, Hercules, CA, USA) at 490 nm.
Bmp 2
Manufactured by Elabscience
Sourced in United States, China
The BMP-2 is a laboratory instrument designed for the detection and quantification of Bone Morphogenetic Protein 2 (BMP-2) in biological samples. It utilizes an enzyme-linked immunosorbent assay (ELISA) technique to measure BMP-2 levels with high sensitivity and specificity.
Automatically generated - may contain errors
Lab products found in correlation
Vegfa, by Elabscience (1 mentions)
Fibroblast growth factor 2 (fgf2), by Elabscience (1 mentions)
Microplate reader, by Bio-Rad (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
3 4 5 dimethylthiazol 2 yl 5 3 carboxymethoxyphenyl 2 4 sulfophenyl 2h tetrazolium mts, by Merck Group (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
β glycerophosphate, by Merck Group (1 mentions)
2 protocols using bmp 2
1
Quantification of Secreted Growth Factors
2
Osteogenic Potential of Eurycoma longifolia
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Mouse calvariae origin osteoblastic cell line subclone 4 (CRL-2594) was purchased from American Type Culture Collection (ATCC) Cell Bank (Manassas, VA, USA). Cell culture reagents- Alpha modified minimal essential medium (α-MEM)), penicillin, streptomycin and fetal bovine serum were sourced from Gibco Laboratories (Grand Island, New York, USA). Ascorbic acid, β-glycerophosphate and MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H tetrazolium) dyes were purchased from Sigma–Aldrich, USA. Eurycoma longifolia Jack (EL) was sourced from Faculty of Pharmacy, University Sains Malaysia (Malaysia). Enzyme-linked immunosorbent assay (ELISA) kits for the expression of ALP, Runx-2, AR, OPN, OCN, BMP-2, collagen type 1, and TGF-β were purchased from Elabscience Biotechnology Co., Ltd. (Wuhan, China). All other chemicals used were of analytical grade and were sourced from pharmacology and cell culture laboratories of Universiti Kebangsaan Malaysia (Malaysia).
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