Caov 3

The human colorectal HCT 116 (CVCL_0291), Caco-2(CVCL_0025), HT-29 (CVCL_0320), SW480 (CVCL_0546), SW620 (CVCL_0547), breast MCF-7(CVCL_0031), T-47D (CVCL_0553), MDA-MB-231(CVCL_0062), ovarian Caov-3(CVCL_0231), SK-OV-3(CVCL_0532) cancer cell lines and HEK293T(CVCL_0063) were obtained from the GeneChem Corporation (Shanghai, China). OVCAR-3(CVCL_0465) was obtained from the Nanjing KeyGen Biology (Nanjing, China). All human cell lines have been authenticated using STR profiling. OVCAR-3, Caco-2, MCF-7, Caov-3 and HEK293T cells were cultured in DMEM (Gibco, Carlsbad, CA, USA). T-47D and OVCAR-3 cells were cultured with RPMI 1640 (Gibco, Carlsbad, CA, USA). HCT 116 and HT-29 cells were cultured with McCoy’s 5a (Gibco, Carlsbad, CA, USA). SW480, SW620, and MDA-MB-231 cells were cultured with Leibovitz’s L-15 (Gibco, Carlsbad, CA, USA) medium containing 10% fetal bovine serum, 100 U/mL penicillin, and 100 mg/mL streptomycin. To inhibit NF-κB activities, BAY 117082 (Selleckchem, Houston, TX, USA) was used.

A2780 and A2780cisR (cisplatin resistant) human ovarian cell lines were gifts from Fudan University Shanghai Cancer Center. IOSE‐80 and HEY‐T30 ovarian cell lines originated from a gift from Dr Luopei Guo (Obstetrics and Gynecology Hospital of Fudan University). ES‐2, SKOV3, OVCAR3, and CAOV3 ovarian cell lines were purchased from GeneChem (Shanghai, China). All cell lines were cultured according to ATCC guidelines at 37°C in a 5% CO2 incubator. The olaparib (Selleck, catalog number S1060) was dissolved in DMSO, and the final concentration of DMSO in the medium was 0.1%. After the cells were plated for 24 h, cells were overlaid with (0, 2, 10, 25, 50 μM) olaparib‐conditioned medium and harvested for 24 h.