Trypsin na2edta

Manufactured by Biosera

Sourced in France

Trypsin-Na2EDTA is a cell dissociation reagent used to detach adherent cells from a culture surface. It is a mixture of the proteolytic enzyme trypsin and the chelating agent sodium EDTA. This combination aids in the disruption of cell-to-cell and cell-to-substrate adhesions, allowing for the effective dissociation of cell monolayers.

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Lab products found in correlation

Phosphate buffered saline (pbs), by Biosera (2 mentions) Penicillin, by Biosera (2 mentions) Streptomycin, by Biosera (2 mentions) L glutamine, by Biosera (2 mentions) Ag1478, by Merck Group (1 mentions) Collagen type 1, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Biosera (1 mentions) Cytarabine, by Merck Group (1 mentions) 17b estradiol e2, by Merck Group (1 mentions) Endothelial cell growth medium mv c 22020, by PromoCell (1 mentions) Dulbecco s modified eagle s medium dmem, by Biosera (1 mentions)

Spelling variants of this product

Trypsin (16 citations)

2 protocols using trypsin na2edta

Cell Culture and Reagents for EGFR Signaling

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Dulbecco’s modified Eagle medium (DMEM), fetal bovine serum (FBS), PBS, trypsin- Na₂EDTA, L-glutamine, penicillin and streptomycin were all obtained from Biosera LTD (Courta-boeuf CEDEX, France). FN was supplied by BD Biosciences (NJ, USA). Type I collagen was supplied by Sigma-Aldrich (Steinheim, Germany). E2 and allosteric inhibitor of EGFR, AG1478, were supplied by Sigma Chemical Co. (St. Louis, MO, USA). All other chemicals used were of the best commercially available grade.

Kyriakopoulou K., Riti E., Piperigkou Z., Koutroumanou Sarri K., Bassiony H., Franchi M, & Karamanos N.K. (2020). ΕGFR/ERβ-Mediated Cell Morphology and Invasion Capacity Are Associated with Matrix Culture Substrates in Breast Cancer. Cells, 9(10), 2256.

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Endothelial Cell Culture Protocol

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Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), PBS, trypsin-Na 2 EDTA, L-glutamine, penicillin, and streptomycin were all obtained from Biosera LTD (Courtaboeuf Cedex, France). Endothelial cell growth medium MV (C-22020) was purchased from PromoCell GmbH (Heidelberg, Germany). The cytostatic agent cytarabine was supplied by Sigma Chemical Co. (St. Louis, MO, USA). The allosteric inhibitor AG1478 (EGFR) and 17-b estradiol (E2) were supplied by Sigma Chemical Co. (St. Louis, MO, USA). All other chemicals used were of the best commercially available grade.

Kyriakopoulou K., Kefali E., Piperigkou Z., Riethmüller C., Greve B., Franchi M., Götte M, & Karamanos N.K. (2022). EGFR is a pivotal player of the E2/ERβ - mediated functional properties, aggressiveness, and stemness in triple-negative breast cancer cells. The FEBS journal, 289(6).

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