Aerrane isoflurane usp

All dogs were allowed to rest for 30 min before any medication. Dogs were randomly assigned to two treatment groups, premedication with DEX intramuscularly (Dexdomitor, Orion Pharma, Finland; 0.5 mg/mL) at 5 μg/kg (DEX5 group) or 10 μg/kg (DEX10 group). After the onset of sedation, an intravenous catheter was placed in the cephalic vein, and generalized anesthesia was induced with tiletamine-zolazepam (3 mg/kg, intravenously, Zoletil^®, Virbac, France; Zoletil powder diluted with 5 mL of sterile water for injection; 50 mg/mL tiletamine and 50 mg/mL zolazepam) until loss of jaw tone. Then, endotracheal intubation was performed, and anesthesia was maintained with inhaled isoflurane (AERRANE isoflurane USP, Baxter, USA) in 100% oxygenation using a rebreathing circuit. Lactate Ringer’s solution (Lactated Ringer’s injection, GHP, Thailand) was intravenously administered at 10 mL/kg/h. All dogs were given analgesic drugs intravenously before surgery.
After the completion of the surgical procedure, all dogs were injected intravenously with 0.1 mg/kg of atipamezole hydrochloride (Antisedan, Orion Pharma, Finland; 5 mg/mL).

After 8 weeks of feeding, the rats were bled by cardiac puncture under Izoflurane (Aerrane Isoflurane USP, Baxter, Poland) general anesthesia. Subsequently, the entire large intestine of each rat was collected. A piece from each of the three parts of the large intestine (cecum, colon, and rectum) was collected and frozen together in liquid nitrogen for Western blot analysis or fixed in 10% buffered formalin, designed for histopathology, histomorphometry, and immunohistochemistry analysis. The other parts were immediately frozen in liquid nitrogen and stored at −80 °C until biochemical analysis.