IL-1β and IL-18 levels in the cell culture medium were detected by using a Human IL-1β High Sensitivity ELISA kit (EK101BHS-01, Multi Science, China) and Human IL-18 ELISA kit (EK118-01, Multi Science, China), according to the manufacturer’s instructions. The optical density was measured at a wavelength of 450 nm, while measurement at 570 nm were screened as an internal control.
Human il 1β high sensitivity elisa kit
Manufactured by MultiSciences Biotech
Sourced in China
The Human IL-1β High Sensitivity ELISA kit is a quantitative assay designed to measure the concentration of human interleukin-1 beta (IL-1β) in biological samples. It utilizes the enzyme-linked immunosorbent assay (ELISA) technique to provide accurate and reliable results.
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3 protocols using human il 1β high sensitivity elisa kit
1
ELISA-based Quantification of IL-1β and IL-18
2
Quantifying Ovarian Cytokines and Hormones
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The level of TNF-α in ovarian tissues was determined using a mouse TNF-α high-sensitivity ELISA kit (MultiSciences Biotech, Hangzhou, China). The level of IL-1β in ovarian tissues and the culture supernatant of KGN cells were analyzed by a mouse or a human IL-1β high-sensitivity ELISA kit (MultiSciences Biotech). The levels of DHEA and testosterone in the culture supernatant of KGN cells were quantified by a DHEA ELISA kit (USCN Life Science) and a testosterone ELISA kit (USCN Life Science), respectively.
3
Quantification of IL-1β and IL-18 Levels
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The IL‐1β and IL‐18 in cell cultured supernatant solution and animals' serums were detected by Human IL‐1β High Sensitivity ELISA Kit (EK101BHS‐01, Multi Science, Hangzhou, China) and Human IL‐18 ELISA Kit (EK118‐01, Multi Science, Hangzhou, China) according to the manufacturer's protocols. The optical density was measured at a wavelength of 450 nm, while measurement at 570 nm were screened as an internal control.
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