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10 protocols using k2so4

1

Characterization of Boron Nitride Nanosheets

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Trimethyl borate (B(OMe)3) was
purchased from ACROS Organics. NH3 (Linde), N2 (Merck), and Ar (Merck) gases were used. The salt concentration
during the measurements was adjusted by analytical-grade salts such
as NaCl, KCl, CsCl, MgCl2·6H2O, K2SO4, K3[Fe(CN)6] (VWR), and LaCl3 (Alfa Aesar), and they were used without further purification.
The pH of the suspensions was adjusted by HCl (VWR) and NaOH (VWR).
All measurements were carried out at 25 °C and a pH value of
7.0 ± 0.5, unless otherwise noted. High-purity water obtained
from the VWR Purity TU + machine was used for all sample preparations.
Water and all of the prepared salt solutions were filtered with a
0.1 μm syringe filter (Millex) prior to use to avoid dust contamination.
The concentration of the BNNS particles was always kept at 5 mg/L
in the light scattering measurements.
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2

Culturing Arthrospira platensis PCC7345

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A. platensis PCC7345 was obtained from the Pasteur Culture Collection (PCC, Paris, France). Stock cultures were grown in a sterile filtrated modified Zarrouk medium containing (g/L): 16.8 NaHCO3 (99.5%, VWR Chemicals, Darmstadt, Germany), 0.5 K2HPO4 (99%, Carl Roth, Karlsruhe, Germany), 2.5 NaNO3 (99.9%, VWR chemicals, Darmstadt, Germany), 1.0 K2SO4 (99.5%, VWR chemicals, Darmstadt, Germany), 1.0 NaCl (99%, Carl Roth, Karlsruhe, Germany), 0.2 MgSO4∙7H2O (99%, Carl Roth, Karlsruhe, Germany), 0.04 CaCl2∙2H2O (99%, Carl Roth, Karlsruhe, Germany) [11 (link)], and 100 µL/L of Hutner’s trace element solution [49 ]. A. platensis stock cultures were maintained in 300 mL Erlenmeyer flasks at 26 °C, 150 rpm, and 75 µmol/m2s fluorescent light (light/dark cycles of 16/8 h, WB750, Mytron Bio- und Solartechnik GmbH, Heilbad Heiligenstadt, Germany). Liquid stock cultures were sub-cultivated every two weeks to prevent aging and cell death.
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3

Hydrothermal Synthesis of KWO Sensors

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Example 13

KWO is grown using the hydrothermal method. [19, 20] A precursor solution containing Na2WO4.2H2O (95%, Alfa Aesar), oxalic acid dihydrate (>99%, VWR), K2SO4 (>99%, VWR), and HCl (36-38%, Aqua Solutions Inc.) was made. This solution was then put into a 30 mL autoclave for synthesis. KWO samples were grown at 160, 180, 210, and 225° C. for 24 hours. Samples used for acetone sensing tests were applied to a sensor slide by blade coating and then annealed at 350° C.

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4

Inorganic Anion Solution Preparation and TA/2-HTA Dosing

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KOH, KI, I2, KHCO3, KCl, KNO3, KH2PO4, K2HPO4, and K2SO4 were purchased from VWR International (USA). Stock solutions of inorganic anions (0.1 M) were prepared in deionized (DI) water for carbonate. The starch solution was prepared by adding 0.5 g of starch (VWR International) in 100 mL of boiling DI water and mixing until complete dissolution. After 12 h settling, the supernatant was collected. TA (0.5 mM) and 2-HTA (0.125 mM) solutions were prepared by dosing 16.72 mg of TA (Sigma-Aldrich, USA) and 4.69 mg of 2-HTA (Sigma-Aldrich) in 200 mL of DI water, adjusting pH to 7.9 with KOH, and mixing on a magnetic stir plate overnight.
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5

Fatty Acid and Tocopherol Analysis Protocol

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Supelco 37 component FAME mix and the standards of the volatile compounds propanediol, α-phellandrene, camphene, myrcene, p-cymene, and limonene were purchased from Sigma-Aldrich (Prague, Czech Republic). Tocotrienol and the mixed solution of tocopherol standards were from ChromaDex (Los Angeles, CA, USA). Kjeldahl tablets containing 3.5 g K2SO4 and 3.5 mg Se, sodium hydroxide (NaOH), n-hexane of PESTINORM® grade, and methanol HiPerSolv CHROMANORM grade (MeOH) were purchased from VWR International (Stříbrná Skalice, Czech Republic). Tashiro’s indicator was from P-LAB (Prague, Czech Republic). Nitric acid (HNO3), sulphuric acid (H2SO4), benzene, isopropanol, and petroleum ether of analytical grade were from Lach-Ner (Neratovice, Czech Republic). Sea sand was purchased from PENTA (Prague, Czech Republic). Ultrapure HPLC water was acquired from Simplicity UV system (Merck Millipore, KGaA, Darmstadt, Germany).
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6

Enzyme-Catalyzed Ring-Opening Polymerization

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5-Hydroxymetyl-2-furancarboxylic acid (HMFA, >97%), was acquired from Carbosynth Compton, Berkshire, UK). ε-Caprolactone (ECL, ≥98%), tetrahydrofuran (THF, >99%), toluene (>99%), chloroform (CHCl3, >99%), dichloromethane (CH2Cl2, ≥99,8%), acetonitrile (>99%), sodium sulphate (Na2SO4, >99%) were from Sigma Aldrich (Steinheim am Albuch, Germany). Magnesium chloride (MgCl2, 98%) and potassium carbonate (K2CO3, 99%) were purchased from Chimopar S.A. (Bucharest, Romania), while sodium chloride (NaCl, >99%) and potassium sulfate (K2SO4, 99%) were products of VWR Chemicals (Leuven, Belgium) and Alfa Aesar GmbH & Co KG (Karlsruhe, Germany), respectively. All chemicals were used as purchased, without further purification.
Three commercially available immobilized enzymes were utilized as biocatalysts for the polymerization reactions: Novozyme 435 (lipase from Candida antarctica B immobilized on acrylic resin) and Lipozyme CalB, (from C. antarctica) were products of Novozymes (Bagsværd, Denmark), while GF-CalB-IM (lipase B from C. antarctica immobilized on microporous ion exchange resin) was purchased from GenoFocus (Daejeon, South Korea). Lipase from porcine pancreas (Sigma-Aldrich, Steinheim, Germany) was used for the enzymatic degradation studies.
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7

Analytical Procedure for Metabolites

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UA, hypoxanthine, xanthine, creatinine, allantoin, urea, NaCl, KH 2 PO 4 , K 2 HPO 4 X3H 2 O, MgSO 4 X7H 2 O, KOH and LiOH were purchased from Sigma-Aldrich (St. Louis, MO). K 2 SO 4 , acetic acid, ammonium acetate, ACN and LC glass vials were purchased from VWR International, Oslo, Norway. All solvents and chemicals were of HPLC grade or higher.
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8

Analytical Procedure for Chitin Analysis

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Petroleum ether (bp. 40 -60 °C, technical), H2SO4 (95 %, technical), K2SO4, CuSO4.5H2O, NaOH, and H3BO3 were all supplied by VWR Chemicals. Tin pressed capsules (8 mm 𝗑 5 mm, standard clean) and all reagents required for the Dumas method were purchased from OEA Laboratories Ltd. Helium was supplied by Nippon Gases. The analytical standard of acetanilide was provided by Thermo Fisher. Chitin (practical grade), HCl (37.0 %), 9-fluorenylmethyl chloroformate (FMOC-Cl), D-glucosamine hydrochloride (analytical standard), and amino acid standards were purchased from Sigma Aldrich. Syringe filters (Chromafil PET 0.20 µm) and Soxhlet thimbles were supplied by Macherey-Nagel. ULC-MS grade acetonitrile and formic acid were bought from Biosolve. The derivatisation reagent for amino acid analyses (6aminoquinolyl-N-hydroxysuccinimidyl carbamate, AQC) was supplied by Waters Co.
Ultrapure water was obtained through the Arium Pro® UV from Sartorius. All reagents were of analytical grade unless specified otherwise.
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9

Fabrication of Nanocomposite Electrodes

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Monomer 3,4-ethylenedioxythiophene (EDOT), poly(sodium4-styrenesulfonate) (PSS) and multiwalled carbon nanotubes—MWCNTs (≥98% carbon basis, O.D. × I.D. × L 10 nm ± 1 nm × 4.5 nm ± 0.5 nm × 3-~6 µm) were purchased from Sigma–Aldrich (Merck KGaA, Darmstadt, Germany) and used as received. The graphene oxide suspension (6.8 mg/mL) was prepared via modified Hummers method (supplied by Institute of Electronic Materials Technology (ITME), Warsaw, Poland). Inorganics: H2SO4 (98 wt.%), HNO3 (65 wt.%), K2SO4, (Avantor Performance Materials Poland S.A., Gliwice, Poland) were of analytical grade and used without any further purification. Electrolytes were prepared using deionised water.
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10

Zeolite Reaction Solution Preparation

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The solutions used to react with the zeolite were prepared using analytically pure Ca(OH)2, NaOH, KOH and K2SO4 (Avantor, Gliwice, Poland). The simulated pore solutions were prepared with deionized water.
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