Human osteoactivin gpnmb anti goat

Manufactured by R&D Systems

The Human Osteoactivin/GPNMB anti-goat is a laboratory reagent used in research applications. It is an antibody that specifically binds to the human osteoactivin/GPNMB protein. This product can be used in various immunological techniques to detect and study the target protein.

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Lab products found in correlation

Tfe3 anti rabbit, by Merck Group (3 mentions) Axio scan z1 slide scanner, by Zeiss (2 mentions) Melan a, by Abcam (1 mentions) Cytokeratin 7, by Abcam (1 mentions) Cathepsin k, by Abcam (1 mentions) Pan cytokeratin, by Novus Biologicals (1 mentions) Hematoxylin, by Vector Laboratories (1 mentions) Mouse anti mitf, by Merck Group (1 mentions) Rabbit anti tfeb, by Fortis Life Sciences (1 mentions) Dq green bsa, by Thermo Fisher Scientific (1 mentions) Lysotracker dnd 99 l 7528, by Thermo Fisher Scientific (1 mentions) Mouse anti lamp1, by Santa Cruz Biotechnology (1 mentions) Fetal bovine serum (fbs), by Euroclone (1 mentions) Dulbecco modified eagle medium (dmem), by Euroclone (1 mentions)

4 protocols using human osteoactivin gpnmb anti goat

Immunohistochemical Analysis of Kidney Cancer

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Hematoxylin and eosin staining was performed by standard methods. Histology was reviewed by a pathologist experienced in evaluating kidney cancer. Immunohistochemistry for TFE3 and GPNMB was performed as previously described [28 ]. Primary antibodies were as follows: Human Osteoactivin/GPNMB anti goat (R&D Systems; 1:800); TFE3 anti rabbit (Sigma Aldrich; 1:800). Spectral karyotyping and TFE3 was performed as previously described [29 (link)] and outlined in Supplementary Methods.

Lang M., Schmidt L.S., Wilson K.M., Ricketts C.J., Sourbier C., Vocke C.D., Wei D., Crooks D.R., Yang Y., Gibbs B.K., Zhang X., Klumpp-Thomas C., Chen L., Guha R., Ferrer M., McKnight C., Itkin Z., Wangsa D., Wangsa D., James A., Difilippantonio S., Karim B., Morís F., Ried T., Merino M.J., Srinivasan R., Thomas C.J, & Linehan W.M. (2023). High-throughput and targeted drug screens identify pharmacological candidates against MiT-translocation renal cell carcinoma. Journal of Experimental & Clinical Cancer Research : CR, 42, 99.

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Immunohistochemical Profiling of Tumor Samples

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Five-micron thick formalin-fixed paraffin-embedded sections were cut from all available tumor blocks. IHC staining and H&E staining were performed by either VitroVivo Biotech (Rockville, MD) or the Molecular Histopathology Laboratory at the Frederick National Laboratory for Cancer Research (Frederick, MD) using standardized methodologies. IHC staining was performed using either Human Osteoactivin/GPNMB anti-goat (R&D Systems, Minneapolis, MN), TFE3 anti-rabbit (Sigma Aldrich, Burlington, MA), Phospho-S6 Ribosomal Protein (Ser235/236) anti-rabbit (Cell Signaling Technology, Danvers, MA), or Phospho-4-E-BP1 (Thr37/46) anti-rabbit (Cell Signaling Technology, Danvers, MA). For selected tumors, an immunohistology panel was performed evaluating HMB45 (Agilent Dako, Carpinteria, CA), Melan-A (Abcam, Waltham, MA), Pan-cytokeratin (Novus Biologicals, Centennial, CO), Cytokeratin 7 (Abcam, Waltham, MA), Cathepsin K (Abcam, Waltham, MA), and Carbonic anhydrase IX (Cell Signaling Technology, Danvers, MA). Images were captured using an AxioScan.Z1 Slide Scanner (Zeiss, Oberkochen, DE).

Schmidt L.S., Vocke C.D., Ricketts C.J., Blake Z., Choo K.K., Nielsen D., Gautam R., Crooks D.R., Reynolds K.L., Krolus J.L., Bashyal M., Karim B., Cowen E.W., Malayeri A.A., Merino M.J., Srinivasan R., Ball M.W., Zbar B, & Marston Linehan W. (2023). PRDM10 RCC: A Birt-Hogg-Dubé-like Syndrome Associated With Lipoma and Highly Penetrant, Aggressive Renal Tumors Morphologically Resembling Type 2 Papillary Renal Cell Carcinoma. Urology, 179.

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Immunohistochemistry of Melanoma Markers

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Five-micron thick formalin-fixed paraffin-embedded sections were deparaffinized and blocked with methanol containing 30% H₂O₂. Antigen retrieval was performed with Citrate buffer at pH 6.0 in a steamer cooker. Slides were blocked in PBS with normal rabbit serum, incubated with primary antibodies overnight and with secondary antibodies conjugated with biotin for 40 min at room temperature. Signal was detected with streptavidin-HRP for 40 min at room temperature. DAB (3,3′-diaminobenzidine) counterstaining was performed by diluting DAB 1:40 in DAB buffer plus H₂O₂ for 2 min and Hematoxylin for 10 seconds (Vector Laboratories). Pictures were obtained with an AxioScan.Z1 Slide Scanner (Zeiss, Oberkochen, DE). The primary antibodies were used as follows: Human Osteoactivin/GPNMB anti goat (R&D Systems, Minneapolis, MN; 1:800), MITF anti mouse (EMD Millipore, Billerica, MA;1:50); TFE3 anti rabbit (Sigma Aldrich; 1:800), TFEB anti rabbit (Bethyl Laboratories, Montgomery, TX; 1:200).

Lang M., Vocke C.D., Ricketts C.J., Metwalli A.R., Ball M.W., Schmidt L.S, & Linehan W.M. (2020). Clinical and Molecular Characterization of Microphthalmia-Associated Transcription Factor (MITF)-Related Renal Cell Carcinoma. Urology, 149, 89-97.

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Quantifying Lysosomal Activity in Cells

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Reagents were obtained from the following sources: human TFEB (4240) anti‐rabbit from Cell Signaling Technology; antibody to murine TFEB from Bethyl laboratories (A303‐673A); human TFE3 anti‐rabbit from Sigma Aldrich (#SAB4200803), antibody to Cadherin 16 from Novus Biologicals (NBP1‐59248); human osteoactivin/GPNMB antigoat from R&D Systems; mouse anti‐LAMP1 was from Santa Cruz (1D4B). DMEM, RPMI, and fetal bovine serum (FBS) were from Euroclone; lentiviral plasmids sh‐RNAs, amino acids, and blasticidine were from Sigma Aldrich; Torin 1 (cat. no. 4247) from Tocris; LysoTracker DND99 (L7528) and DQ Green BSA (D12050) were from Thermo Fisher; siRNAs were purchased as SMART pool from Dharmacon.

Di Malta C., Zampelli A., Granieri L., Vilardo C., De Cegli R., Cinque L., Nusco E., Pece S., Tosoni D., Sanguedolce F., Sorrentino N.C., Merino M.J., Nielsen D., Srinivasan R., Ball M.W., Ricketts C.J., Vocke C.D., Lang M., Karim B., Lanfrancone L., Schmidt L.S., Linehan W.M, & Ballabio A. (2023). TFEB and TFE3 drive kidney cystogenesis and tumorigenesis. EMBO Molecular Medicine, 15(5), e16877.

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