Poly lactic co glycolic acid plga

Poly (lactic-co-glycolic acid) (PLGA, Mw = 44 kDa) with a 50/50 ratio for the lactide to glycolide were purchased from Jinan Daigang Biomaterial Co., Ltd (Jinan, China). Spherical nano-HA particles (100–150 nm) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).

1, 1, 1, 3, 3, 3 hexafluoro 2-propanol (HFIP) was provided by Shanghai Darui Finechemical (Shanghai, China). Poly(lactic-co-glycolic) acid (PLGA, molar ratio of PLA: PGA = 75:25) was provided by Jinan Daigang Biomaterial (Jinan, China). Competent cells, chemicals and kits were provided by Sangon Biotech (Shanghai, China). Restriction enzymes were provided by Thermo scientific (Waltham, MA, USA). Phusion PCR Master Mix and T4 ligase were provided by New England Biolabs (Beijing, China). Human bone mesenchymal stem cells (HBMSCs) were provided by Shanghai Institute of Biochemistry and Cell Biology (SIBCB, CAS, Shanghai, China). They are commercialized cell lines. Dimethyl sulfoxide (DMSO) was provided by Changshu Hong sheng Chemical Reagent (Suzhou, China). Paraformaldehyde (POM) and Cell Counting Kit -8 (CCK-8) were provided by Biyotime Institute of Biotechnology (Haimen, China). Dulbecco’s Modified Eagle’s Medium (DMEM), fetal bovine serum (FBS) and phosphate-buffered saline (PBS) were provided by Mesgen Company (Shanghai, China). Penicillin/streptomycin and trypsin were provided by Shanghai Yuanxiang Medical Equipment (Shanghai, China). A Milli-Q Plus 185 water purification system (Millipore, Burlington, MA, USA) was utilized to produce ultrapure water (18 MΩ cm).