Model 610a

Manufactured by Thermo Fisher Scientific

The Model 610A is a laboratory instrument designed for the measurement and analysis of various samples. It features advanced technology and precision engineering to deliver accurate and reliable results. The core function of this product is to provide users with the necessary tools to conduct various analytical procedures in a laboratory setting.

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Lab products found in correlation

Model 140c hplc system, by Thermo Fisher Scientific (2 mentions) Gas phase sequencer model 492, by Thermo Fisher Scientific (1 mentions)

2 protocols using model 610a

Proteomic Analysis of VE-Cadherin Cleavage

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Recombinant VE-cadherin was exposed to purified LasB and reaction products were electrophoresed, transferred on PVDF membrane and stained with Coomassie. The 50 kDa band was excised and subjected to N-terminal sequencing. Amino acid sequence determination based on Edman degradation was performed using an Applied Biosystems gas-phase sequencer model 492. Phenylthiohydantoinamino acid derivatives generated at each sequence cycle were identified and quantitated on-line with an Applied Biosystems Model 140C HPLC system using the data analysis system for protein sequencing from Applied Biosystems Model 610A. Chromatography was used to identify and quantify the derivatized amino acid removed at each sequence cycle. Retention times and integration values of peaks were compared to the chromatographic profile obtained for a standard mixture of derivatized amino acids

Golovkine G., Faudry E., Bouillot S., Voulhoux R., Attrée I, & Huber P. (2014). VE-Cadherin Cleavage by LasB Protease from Pseudomonas aeruginosa Facilitates Type III Secretion System Toxicity in Endothelial Cells. PLoS Pathogens, 10(3), e1003939.

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Protein Sequencing Using Edman Degradation

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Purified peptides were subjected to Edman degradation on a gas-phase sequencer model ABI 492 (Applied Biosystems, CA, USA). The phenylthiohydantoin (PTH) amino acid derivatives generated at each sequence cycle were identified and quantified on-line with an Applied Biosystems Model 140C
HPLC system using the Applied Biosystems Model 610 A data analysis system for protein sequencing.
The PTH-amino acid standard kit was used and reconstituted according to the manufacturer's instructions. The procedures and reagents were used as recommended by the manufacturer.

Touchard A., Mendel H., Boulogne I., Herzig V., Emídio N.B., King G.F., Charles L., Jaquillard L., Béroud R., Waard M.D., Delalande O., Déjean A., Muttenthaler M., & Duplais C. (2020). Heterodimeric insecticidal peptide provides new insights into the molecular and functional diversity of ant venoms.

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