Kyse 150

Manufactured by Merck Group

Sourced in United States

The KYSE-150 is a laboratory equipment product. It is used for cell culture applications. The KYSE-150 provides a controlled environment for the growth and maintenance of cells in vitro.

Automatically generated - may contain errors

Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Eca109, by Merck Group (2 mentions) Het 1a, by American Type Culture Collection (2 mentions) Kyse30, by Merck Group (2 mentions) 5 azacitidine, by Merck Group (1 mentions) Dnmt1 sirna, by RiboBio (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Eca 109, by Thermo Fisher Scientific (1 mentions) Kyse 150, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Mir 124 3p mimic, by RiboBio (1 mentions) Penicillin and streptomycin, by Thermo Fisher Scientific (1 mentions) Thp 1, by American Type Culture Collection (1 mentions) Phorbol myristate acetate (pma), by Merck Group (1 mentions) Thp 1, by Merck Group (1 mentions) Kyse 410, by Merck Group (1 mentions) Kyse 150, by American Type Culture Collection (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Eca109, by American Type Culture Collection (1 mentions)

6 protocols using kyse 150

Esophageal Cell Line Maintenance and Manipulation

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Human normal esophageal cell line HEEC (Het-1A, ATCC® CRL-2692™) was obtained from the America Type Culture Collection in May 2017, and two human esophageal carcinoma cell lines, KYSE-150 (TCHu236) and Eca-109 (TCHu69), were obtained from Shanghai Institute of Biological Sciences, Chinese Academy of Sciences in May 2017. These cell lines have been authenticated by short-tandem repeat analyses. They are free of mycoplasma contamination.
The cells were maintained in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) supplemented with 10% FBS (Gibco, Grand Island, NY, USA) and 1% Penicillin-Streptomycin (10,000 U/mL) (Invitrogen). For cell transfection, siRNAs including DNMT1 siRNA, BCAT1 siRNA, scrambled siRNA (NC siRNA), miR-124-3p mimics and its negative control (NC mimics) were supplied by Ribobio Company (Guangzhou, China) and pcDNA-BCAT1(OE-BCAT1) or pcDNA-negative (OE-NC) were constructed using pcDNA3.1(+) vector. KYSE-150 and Eca-109 cells grown in 6-well cell plates (1 × 10⁶) were transfected with siRNA or pcDNA3.1vector using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instruction. After 6 h, the transfection solution was replaced by complete medium.
For drug treatment, KYSE-150 or Eca109 cells were treated with 5 μM 5-Azacitidine (5-Aza, Sigma-Aldrich, Saint Louis, MO, USA), which is the inhibitor of DNMT1.

Zeng B., Zhang X., Zhao J., Wei Z., Zhu H., Fu M., Zou D., Feng Y., Luo H, & Lei Y. (2019). The role of DNMT1/hsa-miR-124-3p/BCAT1 pathway in regulating growth and invasion of esophageal squamous cell carcinoma. BMC Cancer, 19, 609.

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Esophageal and Monocyte Cell Culture

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The human esophageal epithelial cell line Het‐1A and human monocyte cell line THP‐1 were purchased from ATCC. THP‐1 cells were differentiated into M0 macrophages with PMA (100 nM; Sigma‐Aldrich) for 24 h. The human ESCC cell lines KYSE150, TE‐1, KYSE30, ECA109, and KYSE410 were purchased from the Institute of Biochemistry and Cell Biology of the Chinese Academy of Sciences. All human cell lines were identified by short tandem repeat analysis, and the results of mycoplasma testing were negative. All human cell lines were cultured with 3 mL RPMI‐1640 medium containing 10% FBS (Gibco) with 100 U/mL penicillin and streptomycin (Gibco), and incubated at 37°C and 5% CO₂ in a humidified incubator of 5% CO₂. The harvested media were centrifuged for 3 min at 1200 g, and the supernatant (CM) was collected and deposited at −80°C until use. Medium without cells was cultivated under the same experimental conditions was prepared as a control.

Feng A., He L., Jiang J., Chu Y., Zhang Z., Fang K., Wang Z., Li Z., Sun M., Zhao Z., Shi J., Zhang L., Chen T, & Xu M. (2023). Homeobox A7 promotes esophageal squamous cell carcinoma progression through C‐C motif chemokine ligand 2‐mediated tumor‐associated macrophage recruitment. Cancer Science, 114(8), 3270-3286.

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Esophageal Cancer Cell Line Cultivation

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Human esophageal cancer cell lines (Kyse150, Kyse170, Eca109, and TE1) were purchased from American Type Culture Collection (ATCC). Cells were cultured in RPMI-1640 (Invitrogen, Carlsbad, CA, USA) containing 10% heat-inactivated fetal bovine serum (Invitrogen) in an atmosphere containing 5% CO₂ at 37 °C. For Trichostatin A (TSA) (CAS No. 58880-19-6, Sigma) treatment, Kyse 150 and Kyse170 cells were transfected for 12–24 h, then treated with 300 nM TSA for additional 48 h.

Chen L., Lu J., Xu T., Yan Z., Guo Y., Dong Z, & Guo W. (2022). KTN1-AS1, a SOX2-mediated lncRNA, activates epithelial–mesenchymal transition process in esophageal squamous cell carcinoma. Scientific Reports, 12, 20186.

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Esophageal Cancer Cell Line Maintenance and UBQLN2-siRNA Transfection

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Human ESCC cell lines KYSE-30, KYSE-70, and KYSE-150 were purchased from Sigma-Aldrich Co. (St. Louis, USA). Ec109 cells were purchased from BnBio Inc. (Beijing, China). The normal human esophageal epithelial cell line Het-1A was bought from the American Type Culture Collection (Manassas, USA). Ec109 cells and KYSE-30 cells were maintained in the RPMI-1640 medium. KYSE-150 cells were maintained in the DMEM medium. KYSE-70 cells were maintained in MEM medium. All media were supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. Het-1A was cultured in BEGM™ BulletKit™. Cells were kept at 37°C in a humidified 5% carbon dioxide atmosphere. For UBQLN2-siRNA transfection, UBQLN2 and negative control siRNA were obtained from Sangon Biotech (Shanghai, China). Also, the transfection was performed using Lipofectamine RNAi-Max reagent (Invitrogen, USA) in accordance with the protocol of the manufacturer.

Wang J.L., Mu X.Y., Ma R., Bai X.H., Zhao Z.J, & Wang Y.Y. (2023). Silencing UBQLN2 Enhances the Radiosensitivity of Esophageal Squamous Cell Carcinoma (ESCC) via Activating p38 MAPK. Journal of Oncology, 2023, 2339732.

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Radiation-Induced Cell Line Alterations

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We purchased ESCC human cell lines TE-1 and KYSE-150 from the Shanghai Institute of Cell Research (Shanghai, China). None of the cells had been irradiated by radiation. TE-1 cells were cultured in DMEM medium (Hyclone, USA) and KYSE-150 cells were cultured in RPMI-1640 medium (Hyclone, USA) supplemented with 10% FBS (Biological Industries, Kibbutz Beit-haemek, Israel) at 37°C in 5% CO₂. POLI stably over-expressed KYSE-150 cells was established and kept in our laboratory as previously described.[11 (link)] To generate POLI stably knockdown cell, TE-1 cells were infected with lentivirus containing the POLI-targeted shRNA sequence and selected with 1 μg/ml puromycin (Sigma-Aldrich, USA). The knockdown efficiency was verified by qPCR and western blot. Xenografted tumors and cells were irradiated with X-ray energy at a dose rate of 2 Gy/min and a 6 MeV dose rate by a linear accelerator (Varian, Palo Alto, CA, USA).

Li X., Gao D., Shen F., Chen H., Zhang Z., He C., Gao A., Lang Y., Zhu X., Zhou J., Shang Z.F., Ding W.Q, & Zhu J. (2023). Polymerase iota (POLI) confers radioresistance of esophageal squamous cell carcinoma by regulating RAD51 stability and facilitating homologous recombination. Cell Death Discovery, 9, 291.

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Isolation and Culture of Human LECs

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Human ESCC cell line KYSE-150 and primary human LECs were purchased from Procell (Wuhan, China). T25 culture flasks (707001, NEST) were coated with 0.1 mg/ml poly-L-lysine (PB180522, Procell) at 37°C for one hour. Human LEC were then seeded at a density of 0.5 million cells per flask. KYSE-150 was cultured with 10% FBS RPMI/F12 medium (32160801, Sigma-Aldrich) and human LECs were cultured with ECM medium (CM-H026, Procell) in a humidified incubator at 5% CO₂ and 37°C.

Ma M., Li L., Yang S.H., Huang C., Zhuang W., Huang S., Xia X., Tang Y., Li Z., Zhao Z.B., Chen Q., Qiao G, & Lian Z.X. (2024). Lymphatic endothelial cell-mediated accumulation of CD177+Treg cells suppresses antitumor immunity in human esophageal squamous cell carcinoma. Oncoimmunology, 13(1), 2327692.

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