For the validation experiment with 2PM, the mouse was intravenously injected with 150 µL lectin-FITC (Lycopersicon Esculentum (Tomato) Lectin fluorescein, 2 mg/ml, L32478, Invitrogen, USA) to stain the vasculature with a total circulation time of ~3 min. Following the circulation period, the mouse was euthanized while still under anesthesia. Two-photon imaging was conducted on the extracted mouse brain.
L32478
Manufactured by Thermo Fisher Scientific
Sourced in United States
The L32478 is a laboratory centrifuge designed for general-purpose use. It features a compact and durable construction to accommodate a range of sample volumes and tube sizes. The centrifuge's core function is to separate heterogeneous mixtures into their constituent components through the application of centrifugal force.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using l32478
1
Vascular Staining and 2PM Imaging
2
Lectin-Based Vascular Labeling in Mice
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As reported in the literature,35 (link) after anesthesia, fluorescently labeled Ricinus communis agglutinin I (RCA I, L32478, Invitrogen, US) and Lycopersicon esculentum lectin (LEL, L32471, Invitrogen, US) were injected into mice via the tail vein. One hour later, the mice were perfused with saline containing 1% PFA, and the ovary/mesentery-small intestine tissue was removed and quickly cooled in liquid nitrogen. Then, the tissues were sectioned on a frozen section machine and observed under an Olympus confocal microscope. The thickness of the sections was 10 μm.
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