Ultrascan 1000 2k 2k ccd camera

Manufactured by Ametek

Sourced in United States

The UltraScan 1000 is a 2k×2k CCD camera designed for laboratory applications. It features a high-resolution sensor capable of capturing detailed images. The camera's core function is to provide high-quality image data for scientific analysis and documentation purposes.

Automatically generated - may contain errors

Lab products found in correlation

Tecnai g2 f20 transmission electron microscope, by Thermo Fisher Scientific (5 mentions) Microscopy suite control, by Ametek (2 mentions) Tecnai g2 f30, by Thermo Fisher Scientific (2 mentions) Carbon formvar film, by ProSciTech (2 mentions) 15 s copper grid 400 mesh, by ProSciTech (1 mentions) Lab6 jem2100, by JEOL (1 mentions) Zetasizer nano z, by Malvern Panalytical (1 mentions) 626 cryo holder, by Ametek (1 mentions) Libra 120, by Zeiss (1 mentions) Formvar carbon coated copper grids, by ProSciTech (1 mentions) Microscopy suite software, by Ametek (1 mentions) Microscopy suite, by Ametek (1 mentions)

Spelling variants of this product

Ultrascan 1000 CCD camera (67 citations) Ultrascan 2K CCD camera (19 citations) Ultrascan 1000 CCD (12 citations) Ultrascan 2k × 2k CCD camera (8 citations) UltraScan 2k × 2k CCD (8 citations) 2k×2k CCD camera (8 citations) UltraScan 2k × 2k camera (7 citations) UltraScan 1000 2k × 2k CCD (2 citations) Ultrascan 1000 2k × 2k camera (2 citations) 2×2K Ultrascan 1000 (2 citations)

12 protocols using ultrascan 1000 2k 2k ccd camera

Time-lapse TEM Imaging of hIAPP Aggregation

Check if the same lab product or an alternative is used in the 5 most similar protocols

hIAPP aggregation was examined experimentally by transmission electron microscopy (TEM). For this, full length hIAPP (AnaSpec Inc.) was reconstituted overnight in 100% hexafluoro-2-isopropanol (HFIP) to dissolve all preformed aggregates. The solution was then freeze-dried and a stock solution was prepared in Milli-Q water at a concentration of 25 μM. The peptide solution was allowed to aggregate at room temperature up to 24 h and TEM analysis was performed after 0 min, 30 min, 1 h and 24 h of incubation times. For this, 5 μL of peptide-containing solution was pipetted onto a glow discharged (15 s) copper grid (400 mesh; ProSciTech), followed by 1 min of adsorption. Excess sample was then drawn off using filter paper and the grid was washed by Milli-Q water, with the excess drawn off as previously. The grid was stained with a drop of 1% uranyl acetate for 30 s, then the excess stain was drawn off and the grid was air dried. Imaging was performed by a Tecnai G2 F20 transmission electron microscope (FEI, Eindhoven, The Netherlands) operated at a voltage of 200 kV. Images were recorded using a Gatan UltraScan 1000 (2k×2k) CCD camera (Gatan, California, USA) and Gatan Microscopy Suite control software.

Sun Y., Kakinen A., Xing Y., Pilkington E.H., Davis T.P., Ke P.C, & Ding F. (2018). Nucleation of β-rich Oligomers and β-barrels in the Early Aggregation of Human Islet Amyloid Polypeptide. Biochimica et biophysica acta. Molecular basis of disease, 1865(2), 434-444.

+ Open protocol

+ Expand

Visualizing hIAPP8–20 Oligomerization by TEM

Check if the same lab product or an alternative is used in the 5 most similar protocols

Beta barrel and oligomer formation of hIAPP8–20 were further confirmed using transmission electron microscopy (TEM). For this hIAPP was dissolved in Milli-Q water at 400 µM concentration, incubated up to 48 h at room temperature (20 °C). At specific time points of incubation (i.e., 6 h and 48 h), 5 µL of peptide-containing solution was pipetted onto a glow discharged (15 s) copper grid (400 mesh; ProSciTech), followed by 1 min of adsorption. Excess sample was then drawn off using filter paper and the grid was washed by Milli-Q water with the excess drawn off. The grid was stained with a drop of 1% uranyl acetate for 30 s, then the excess stain was drawn off and the grid was air dried. Imaging was performed by a Tecnai G2 F20 transmission electron microscope (FEI, Eindhoven, The Netherlands) operated at a voltage of 200 kV. Images were recorded using a Gatan UltraScan 1000 (2k×2k) CCD camera (Gatan, California, USA) and Gatan Microscopy Suite control software.

Sun Y., Kakinen A., Xing Y., Faridi P., Nandakumar A., Purcell A.W., Davis T.P., Ke P.C, & Ding F. (2019). Amyloid self-assembly of hIAPP8-20 via the accumulation of helical oligomers, α-helix to β-sheet transition, and formation of β-barrel intermediates. Small (Weinheim an der Bergstrasse, Germany), 15(18), e1805166.

+ Open protocol

+ Expand

Cryo-TEM and DLS Characterization of Micelles

Check if the same lab product or an alternative is used in the 5 most similar protocols

Micelle morphologies were determined from cryo-transmission electron microscopy (cryo-TEM) images. A drop of micelle solution was deposited on Lacey carbon membrane grids. The excess of liquid on the grid was absorbed with a filter paper, and the grid was quench-frozen quickly in liquid ethane to form a thin vitreous ice film. Once placed in a Gatan 626 cryo-holder cooled with liquid nitrogen, the samples were transferred to the microscope and observed at low temperature (−180 °C). Cryo-TEM images were recorded on an Ultrascan 1000, 2k × 2k CCD camera (Gatan, Pleasanton, CA, USA), using a LaB6 JEOL JEM2100 (Jeol, Tokyo, Japan) cryo microscope operating at 200 kV with a JEOL low-dose system (Minimum Dose System, MDS) to protect the thin ice film from any irradiation before imaging and reduce the irradiation during the image capture.
The hydrodynamic diameter and size distribution (polydispersity index, PDI) of the RPN solutions were measured with the dynamic light scattering (DLS) method using a Zetasizer Nano ZS (Malvern Instruments, Worcestershire, UK) at 25 ± 0.1 °C with angle of 173°. Zeta potential was measured by the electrophoretic mobility method with the same apparatus.

Le Guyader G., Do B., Rietveld I.B., Coric P., Bouaziz S., Guigner J.M., Secretan P.H., Andrieux K, & Paul M. (2022). Mixed Polymeric Micelles for Rapamycin Skin Delivery. Pharmaceutics, 14(3), 569.

+ Open protocol

+ Expand

Exosome Characterization by TEM

Check if the same lab product or an alternative is used in the 5 most similar protocols

Exosomes were fixed with 2% glutaraldehyde/PBS for 30 min at room temperature. A volume of 6 μl was applied to a glow-discharged 200-mesh Cu grid coated with carbon-Formvar film (ProSciTech, Kirwan, QLD, Australia) and allowed to absorb for 5 min. Grids were washed twice with Milli-Q water and contrasted with 1.5% uranyl acetate. Transmission electron microscopy (TEM) was performed on a Tecnai G2 F30 (FEI, Eindhoven, NL) TEM operating at 300 kV across × 15,000 to × 36,000 magnification. Electron micrographs were captured with a Gatan UltraScan® 1000 2 k × 2 k CCD camera (Gatan, Pleasanton, CA).

Quek C., Bellingham S.A., Jung C.H., Scicluna B.J., Shambrook M.C., Sharples R.A., Cheng L, & Hill A.F. (2016). Defining the purity of exosomes required for diagnostic profiling of small RNA suitable for biomarker discovery. RNA Biology, 14(2), 245-258.

+ Open protocol

+ Expand

Cryo-TEM Imaging of Liposomes

Check if the same lab product or an alternative is used in the 5 most similar protocols

A total of 5 μL of liposomes, diluted to a concentration of 250 µM in buffer H, were dropped onto a Quantifoil grid (Quantifoil Micro Tools GmbH, Großlöbichau, Germany). After removing the excess liquid from the grid using a filter paper, the grid was immediately frozen in liquid ethane to create a thin vitreous ice layer. This rapid freezing process was accomplished using a custom-made mechanical cryo-plunger. The sample was then inserted into a Gatan 626 cryo-holder, cooled with liquid nitrogen and transferred to the microscope for observation at a low temperature of −180 °C. The images were captured using a LaB6 JEOL JEM2100 cryo-microscope (JEOL, Tokyo, Japan) operating at 200 kV. The microscope was equipped with an UltraScan 1000 2k × 2k CCD camera (Gatan, Pleasanton, CA, USA) and a JEOL low-dose system (Minimum Dose System, MDS, JEOL, Tokyo, Japan) to protect the thin ice film from irradiation both before and during imaging.

Vlieghe A., Niort K., Fumat H., Guigner J.M., Cohen M.M, & Tareste D. (2023). Role of Lipids and Divalent Cations in Membrane Fusion Mediated by the Heptad Repeat Domain 1 of Mitofusin. Biomolecules, 13(9), 1341.

+ Open protocol

+ Expand

Effects of EGCG on IAPP Amyloid Formation

Check if the same lab product or an alternative is used in the 5 most similar protocols

The effects of EGCG on the formation and morphology of IAPP amyloids were investigated using high-resolution TEM. For that solutions of IAPP and IAPP mixed with fresh EGCG, oxidized EGCG, or EGCG reduced by GSH were incubated at room temperature. All chemicals in the test were at 50 μM and the molar ratios of IAPP:EGCG species:GSH were kept at 1:1:1. Approximately 4 μL of each sample solution after 20 min, 1 h, 6 h and 24 h of incubation was pipetted onto a glow discharged (15 s) copper grids (100 mesh; ProSciTech), followed by 60 s of adsorption. Excess samples were then drawn off using filter paper and the grids washed twice by Milli-Q water and excess drawn off as described above. The grids were stained with 2% uranyl acetate for 15 s, with excess stain drawn off, and were air-dried. Imaging was performed by a Tecnai G2 F20 transmission electron microscope (FEI, Eindhoven, The Netherlands), operating at a voltage of 200 kV. Images were recorded using a Gatan UltraScan 1000 (2k×2k) CCD camera (Gatan, California, USA) and Gatan Microscopy Suite control software.

Kakinen A., Adamcik J., Wang B., Ge X., Mezzenga R., Davis T.P., Ding F, & Ke P.C. (2018). Nanoscale inhibition of polymorphic and ambidextrous IAPP amyloid aggregation with small molecules. Nano research, 11(7), 3636-3647.

+ Open protocol

+ Expand

Transmission Electron Microscopy of Exosomes

Check if the same lab product or an alternative is used in the 5 most similar protocols

For TEM analyses, exosome-PBS suspensions from two randomly selected samples, one VEX (Bird tag# BL3825; leghorn VEX) and one TEX (Bird tag# OR1760; leghorn TEX) were floated on to 400 mesh, carbon-coated copper grids that were previously glow discharged with a PELCO easiGlow™ glow discharge system to render the surface of the grids hydrophilic. Grids were then subjected to washes on drops of water, and then negative stained with 2% uranyl acetate. Air-dried grids were examined with a Zeiss Libra 120 transmission electron microscope at 120 kV, and images were acquired with a Gatan Ultrascan 1000 2k × 2k CCD camera in the bioimaging core at the Delaware Biotechnology Institute at the University of Delaware. For each set of analyses, at least 20 fields were imaged and representative images are shown in results sections.

Neerukonda S.N., Tavlarides-Hontz P., McCarthy F., Pendarvis K, & Parcells M.S. (2019). Comparison of the Transcriptomes and Proteomes of Serum Exosomes from Marek’s Disease Virus-Vaccinated and Protected and Lymphoma-Bearing Chickens. Genes, 10(2), 116.

+ Open protocol

+ Expand

Amyloid Fibril Imaging Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

IAPP, IAPP amyloids, aLac and Lys (25 μM; IAPP:proteins =1:1) were incubated in Milli-Q water at 25 °C for 24 h. A 10 μL aliquot was then taken and placed on 400 mesh carbon-coated formvar copper grids (ProSciTech) that were glow-discharged for 15 s to promote hydrophilicity. Sample adsorption was undertaken for 60 s, then drawn off on filter paper. Grids were washed twice in 10 μL Milli-Q water. 5 μL of 1% uranyl acetate (in water) was utilized to twice-stain grids, by touching one droplet and immediately drawing the stain off, and then placing the grid atop the second droplet to stain for 15 s. TEM images were obtained on a Tecnai TF20 transmission electron microscope (FEI) with an UltraScan 1000 (2k × 2k) CCD camera (Gatan).

Pilkington E.H., Xing Y., Wang B., Kakinen A., Wang M., Davis T.P., Ding F, & Ke P.C. (2017). Effects of Protein Corona on IAPP Amyloid Aggregation, Fibril Remodelling, and Cytotoxicity. Scientific Reports, 7, 2455.

+ Open protocol

+ Expand

Quantitative Analysis of IAPP Amyloid Fibrils

Check if the same lab product or an alternative is used in the 5 most similar protocols

Carbon-coated formvar copper grids (400 mesh) were glow-discharged to promote hydrophilicity. Mature IAPP amyloids (final concentration: 0.1 mg/mL) were mixed with FBS at a 1:5 ratio by protein mass, and 10 μL aliquots were taken at 0 min (immediately), 5 min, 30 min, 2 h and 24 h, and allowed to absorb to the grid for 60 s before excess liquid was drawn off using filter paper. Grids washed in 10 μL Milli-Q water and liquid drawn off as previously. Samples were then double-stained with 1% aqueous uranyl acetate by touching the grids to one 5 μL droplet and then drawing liquid off immediately, before placing the grid on a second droplet for 15 s in the dark and subsequently removing excess stain. TEM images were taken on a Tecnai F20 transmission electron microscope (FEI) utilizing an UltraScan 1000 (2k × 2k) CCD camera (Gatan). IAPP amyloid fibril diameters were subsequently analyzed using Gatan Microscopy Suite software, with 100 points taken for each sample set assessed. Gaussian modeling of fibril diameter distribution (least squares fit) was applied using Prism (GraphPad).

Pilkington E.H., Gustafsson O.J., Xing Y., Hernandez-Fernaud J., Zampronio C., Kakinen A., Faridi A., Ding F., Wilson P., Ke P.C, & Davis T.P. (2018). Profiling the Serum Protein Corona of Fibrillar Human Islet Amyloid Polypeptide. ACS nano, 12(6), 6066-6078.

+ Open protocol

+ Expand

Transmission Electron Microscopy of IAPP Fibrils

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sonicated and un-sonicated IAPP amyloid fibrils of different ages were examined using transmission electron microscopy (TEM). For this a 5 μL of amyloid-containing solution was pipetted onto glow discharged (15 s) copper grids (400 mesh; ProSciTech), followed by 1 min of adsorption. Excess samples were then drawn off using filter paper and the grids were washed by Milli-Q water with the excess drawn off. The grids were stained for 30 s with a drop of 1% uranyl acetate, then the excess stain was drawn off and the grid was air dried. Imaging was performed by a Tecnai G2 F20 transmission electron microscope (FEI, Eindhoven, The Netherlands) operated at a voltage of 200 kV. Images were recorded using a Gatan UltraScan 1000 (2k×2k) CCD camera (Gatan, California, USA) and Gatan Microscopy Suite control software.

Kakinen A., Sun Y., Javed I., Faridi A., Pilkington E.H., Faridi P., Purcell A.W., Zhou R., Ding F., Lin S., Chun Ke P, & Davis T.P. (2018). Physical and Toxicological Profiles of Human IAPP Amyloids and Plaques. Science bulletin, 64(1), 26-35.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!