Seratrodast

20(S)-panaxadiol (PD, purity ≥ 98%) was purchased from Shanghai yuanye BioTechnology (Shanghai, China). Antiplatelet agents such as vorapaxar (VP), ticagrelor (TG), and seratrodast (ST) were purchased from MedChemExpress (New Jersey, USA). The kits for measuring activated partial thromboplastin time (APTT), prothrombin (PT), and fibrinogen were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Thrombin time (TT) was purchased from YaJi Biological (Shanghai, China). Hemocoagulase and cyclic adenosine monophosphate (cAMP) ELISA kits were supplied by Jinzhou Ahon Pharmaceutical (Liaoning, China) and Sino Best Biological Technology (Shanghai, China), respectively. FITC-conjugated anti-human CD62P and PAC-1 antibodies were obtained from BioLegend (California, USA). Antibodies against Akt, phospho-Akt (Ser473), PI3K, phospho-PI3K (Tyr607), GSK3β, phospho-GSK3β (Ser9), and β-actin were obtained from Abcam (Cambridge, UK). Platelet function reagents for thrombin (Chrono-Log Corporation, Pennsylvania, USA) and Fluo-3 AM calcium indicators (Beyotime Biotechnology, Shanghai, China) were used in this study.

20(S)-protopanaxadiol (PPD, purity of ≥98% by HPLC) was purchased from Shanghai yuanye Bio-Technology Co., Ltd (Shanghai, China). Hemocoagulase was purchased from Jinzhou Ahon Pharmaceutical Co, Ltd. (Liaoning, China). Prothrombin (PT), activated partial thromboplastin time (APTT), thrombin time (TT), and fibrinogen (FIB) kits were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Luciferin/luciferase reagent and thrombin were purchased from Chrono-Log Corporation (Pennsylvania, USA). Vorapaxar, ticagrelor, and seratrodast were purchased from MedChemExpress (New Jersey, USA). Cyclic adenosine monophosphate (cAMP), cyclic guanosine monophosphate (cGMP), and human protease activated receptor 1 ELISA kits were purchased from Sino Best Biological Technology Co., Ltd. (Shanghai, China). FITC-conjugated anti-human CD62P (P-selectin) and FITC-conjugated anti-human PAC-1 were purchased from BioLegend (California, USA). Fluo-3 AM calcium indicators and lactate dehydrogenase (LDH) cytotoxicity assay kits were purchased from Beyotime Biotechnology (Shanghai, China).