Tumor cells (5 × 105; AsPC-1, MIA PaCa-2) were seeded in six-well plates and incubated with complete medium for 12 h. The medium was then replaced with complete medium (control group), complete medium: SCM = 1:1 (SCM group), or co-cultured with 5 × 105 hSCs or 1 × 106 RSC96 using a Transwell insert (0.4 μm pore size, Corning Inc.) in complete medium and cultures were maintained for 24 h. Thereafter, the 5-Ethynyl-2´-deoxyuridine (EdU) proliferation assay was performed with BeyoClick™ EdU-555 detection kits (Beyotime) according to the manufacturer's instructions.
Beyoclick edu 555 detection kit
Manufactured by Beyotime
Sourced in China
The BeyoClick™ EdU-555 detection kits are a set of laboratory reagents designed to facilitate the detection and analysis of newly synthesized DNA in cells. The kits incorporate the nucleoside analog EdU (5-ethynyl-2'-deoxyuridine), which can be incorporated into DNA during cell division and detected using a fluorescent azide compound.
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Lab products found in correlation
6 protocols using beyoclick edu 555 detection kit
1
Tumor Cell Proliferation Assay
2
Measuring RASF Proliferation Potential
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To evaluate the proliferation viability of RASFs, an EdU assay was conducted using BeyoClick™ EdU-555 detection kits (Beyotime, Shanghai, China). Transfected RASFs were seeded in six-well plates and incubated in complete medium for 24 h. After incubation with 50 mM EdU for 6 h, the cells were fixed and stained for 30 min. Nucleic acid was stained using Hoechst 33342. All images were captured using a fluorescence microscope.
3
Evaluating SGC-7901 Cell Proliferation
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To evaluate the proliferation viability of SGC‐7901 cells, the EdU assay was conducted with a BeyoClick™ EdU‐555 detection kits (Beyotime). Transfected RASFs were seeded in six‐well plates and incubated with complete medium for 24 h. After incubation with 50 mM EdU for 6 h, the cells were fixed and stained for 30 min. The nucleic acid was stained with Hoechst 33342. All images were captured with a fluorescent microscope.
4
Evaluating Cell Proliferation Viability
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The EdU assay was carried out to assess the proliferation viability of cells using a BeyoClickTM EdU-555 detection kit (Beyotime, Shanghai, China). Transfected cells were cultured in 12-well plates and incubated with medium for 12 h. After incubation with EdU for 2 h, the cells were fixed and stained for 30 minutes. Blue-fluorescent nuclear counterstain-Hoechst 33,342 was used to stain the nucleic acid. Images were captured with a fluorescent microscope.
5
Cell Proliferation Viability Assay
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This assay was performed according to the method of a previous study [45 (link)]. To assess the proliferation viability of cells, the EdU assay was carried out with a BeyoClickTM EdU−555 detection kit (Beyotime, Shanghai, China). Transfected RASFs or MH7A cells were seeded in twelve-well plates and incubated with complete medium for 12 h. After incubation with 50 mM EdU for 6 h, the cells were fixed and stained for 30 min. The nucleic acid was stained with Hoechst 33342. All images were captured with a fluorescent microscope (Olympus Optical Co., Ltd., Tokyo, Japan).
6
Quantifying Cell Proliferation with EdU Assay
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To detect cell proliferation, 5-Ethynyl-2’-deoxyuridine (EdU) assays were carried out with the BeyoClickTMEdU-555 detection kit (C0075S, Beyotime, Shanghai, China). The cells were incubated with 50 mM of EdU medium at 37 °C for 2 h. Subsequently, they were fixed with 4% paraformaldehyde for 15 min, washed three times with PBS for 5 min, permeabilized with 0.3% Triton X-100 for 15 min, and incubated with the Click reaction cocktail for 30 min. The nuclei were stained with DAPI. The fluorescence images were captured under a fluorescence microscope. The total number of cells and the number of EdU-positive cells were counted using Image J software (v1.53c).
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