Axio observer a1 microscope

Manufactured by Zeiss

Sourced in Germany, France

The Axio Observer A1 is a high-quality microscope designed for basic research and educational applications. It features a stable and ergonomic stand, allowing for comfortable observation. The microscope provides a range of illumination options, including transmitted and reflected light, to support various imaging techniques. The Axio Observer A1 is a versatile tool that can be configured to meet the needs of a variety of research and educational settings.

Automatically generated - may contain errors

Lab products found in correlation

Mirax scanner, by Zeiss (6 mentions) Axiocam mrc5 camera, by Zeiss (5 mentions) Axiocam mrm camera, by Zeiss (5 mentions) Fluoromount g, by Southern Biotech (4 mentions) Hoechst 33342, by Thermo Fisher Scientific (4 mentions) Affinipure fab fragment goat anti mouse igg h l, by Jackson ImmunoResearch (3 mentions) Axiocam erc5, by Zeiss (3 mentions) Axiovision 4, by Zeiss (3 mentions) Paraformaldehyde (pfa), by Merck Group (3 mentions) Ripa buffer, by Thermo Fisher Scientific (3 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (3 mentions) Propidium iodide, by Thermo Fisher Scientific (3 mentions) Normal goat serum (ngs), by Jackson ImmunoResearch (2 mentions) Fluar 40 oil immersion objective lens 1.3 na, by Zeiss (2 mentions) Dg 4 wavelength switcher, by Teledyne (2 mentions) Metafluor, by Teledyne (2 mentions) Hek293t cell, by American Type Culture Collection (2 mentions) Jetprime transfection reagent, by Polyplus Transfection (2 mentions) Daq usb x series device, by National Instruments (2 mentions) Bond rx, by Leica (2 mentions)

Close spelling variants of this product

Axio Observer A1 fluorescence microscope (27 citations) Axio Observer A1 inverted light microscope (2 citations) Axio Observer A1 inverted microscope (52 citations) Axio-observer A1 fluorescent microscope (6 citations) Axio Observer A1 (540 citations) Observer A1 microscope (28 citations) Axio Observer microscope (539 citations) Axio A1 microscope (19 citations) Observer A1 (61 citations) Axio Observer (1225 citations)

144 protocols using axio observer a1 microscope

Neurotrophic Factor Receptor Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols

HEK293T cells (ATCC, mycoplasma negative) were transfected with a combination of 0.125 µg trkB/trkA/trkC in pcDNA plasmid and 0.125 µg p75NTR (all gifts from Moses Chao, Addgene plasmid #24088, #24089, #24093, and #24091, respectively) using Lipofectamine 2000 (Invitrogen, 11668-019) in a medium containing DMEM (Gibco, 41966-029), 10% FBS, 1% penicillin/streptomycin. Before labeling, cells were incubated in serum-free medium for 1 h. Aliquot of 0.1 µM BDNF^SNAP was coupled to 0.3 µM of SNAP-surface 647 (New England Biolabs, S9136S) for 1 h at 37 °C and applied onto cells for 15 min at 4 °C and imaged using a Zeiss AxioObserver A1 microscope.
DRG from TrkB^CreERT2::RFP mice was collected in PBS and incubated in 1 mg/ml collagenase IV (Sigma-Aldrich, C5138) and 0.05% Trypsin (Gibco, 25300-054) for 25 min each at 37 °C. Cells were filtered and suspended in medium containing DMEM (Gibco, 41966-029), 10% heat inactivated fetal bovine serum (PAA, A15101), 0.8% glucose, and 100 U of penicillin/streptomycin (Gibco, 15140-122). Cells were plated on glass coverslips treated with poly-L-lysine and stored at 37 °C. An equimolar concentration of BDNF^SNAP was coupled to a mixture of SNAP-Biotin (New England Biolabs, S9110S) and QD655 quantum dots (Invitrogen, Q10121MP) at 37 °C for 30 min. Cells were labeled with the above mixture for 5 min and imaged using a Zeiss AxioObserver A1 microscope.

Dhandapani R., Arokiaraj C.M., Taberner F.J., Pacifico P., Raja S., Nocchi L., Portulano C., Franciosa F., Maffei M., Hussain A.F., de Castro Reis F., Reymond L., Perlas E., Garcovich S., Barth S., Johnsson K., Lechner S.G, & Heppenstall P.A. (2018). Control of mechanical pain hypersensitivity in mice through ligand-targeted photoablation of TrkB-positive sensory neurons. Nature Communications, 9, 1640.

+ Open protocol

+ Expand

Zebrafish Caudal Fin Regeneration

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Zebrafish between 3 dpf and 3 weeks post-fertilization (wpf) were anesthetized in 0.02% Tris-buffered Tricaine and then placed on a glass slide on a dissecting microscope stage. The caudal fin (tail) was cut distal to the notochord tip with a scalpel blade. Fish were either frozen individually in microcentrifuge tubes to await molecular analyses, or were housed individually in a 24-well tissue culture dish in E2 embryo media with methylene blue (http://zebrafish.org/documents/protocols.php) such that caudal fin regeneration and growth could be quantified. Fish were examined and photographed using a Zeiss Axio Observer A1 microscope and AxioCam HRc CCD camera. Growth metrics were conducted as outlined in (29 (link)) and all measurements were obtained with the FIJI image processing package of ImageJ (30 (link)). Regeneration levels were determined by comparing images taken immediately and 3 days after amputation of the fin. Statistical analyses were conducted using JMP 11 (SAS) and unless noted, Mann–Whitney unpaired tests were used.

Rahn J.J., Bestman J.E., Stackley K.D, & Chan S.S. (2015). Zebrafish lacking functional DNA polymerase gamma survive to juvenile stage, despite rapid and sustained mitochondrial DNA depletion, altered energetics and growth. Nucleic Acids Research, 43(21), 10338-10352.

+ Open protocol

+ Expand

Live-cell microscopy of cyanobacteria

Check if the same lab product or an alternative is used in the 5 most similar protocols

All live-cell microscopy was performed using exponentially growing cells. Two mL of culture was spun down at 5000xg for 30 s, resuspended in 200 µl of BG-11 and 2 µl transferred to a square 1.5% agarose +BG-11 pad on glass slides. All images were captured using a Zeiss Axio Observer A1 microscope (100x, 1.46NA) with an Axiocam 503 mono camera except the carboxysome induction experiments. Carboxysome induction experiments were performed using a Nikon Ti2-E motorized inverted microscope with LED-based light sources (100x, 1.45NA) with a Photometrics Prime 95B Back-illuminated sCMOS Camera. Image analysis was performed using Fiji v 1.0.

MacCready J.S., Hakim P., Young E.J., Hu L., Liu J., Osteryoung K.W., Vecchiarelli A.G, & Ducat D.C. (2018). Protein gradients on the nucleoid position the carbon-fixing organelles of cyanobacteria. eLife, 7, e39723.

+ Open protocol

+ Expand

COMET Assay for DNA Damage Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

COMET assay was performed as described in Olive and Banáth (2006) (link). Cell lysis and electrophoresis were performed in alkaline conditions. DNA was stained for 20 min at room temperature with 20 μL of a 10 mg/ml Ethidium Bromide solution (ThermoFisher Scientific) in 10 mL of distilled water; then slides were rinsed with distilled water to remove excess stain. Slides were imaged on a Zeiss AXIO Observer A1 microscope. Comet tail moment was measured using the ImageJ Macro “Comet Assay.” Four independent biological replicates of this experiment were performed, three of them at 48 h after transfection and one at 72 h after transfection. Since the results were comparable, the data from the four experiments are shown all together.

Rossi F., Beltran M., Damizia M., Grelloni C., Colantoni A., Setti A., Di Timoteo G., Dattilo D., Centrón-Broco A., Nicoletti C., Fanciulli M., Lavia P, & Bozzoni I. (2022). Circular RNA ZNF609/CKAP5 mRNA interaction regulates microtubule dynamics and tumorigenicity. Molecular Cell, 82(1), 75-89.e9.

+ Open protocol

+ Expand

Live Imaging of G-CaMP Calcium Sensors

Check if the same lab product or an alternative is used in the 5 most similar protocols

Live imaging was performed on a Zeiss Axio Observer A1 microscope. A heated platform was used to maintain temperature at 37 °C. The GFP filter was used to view G-CaMP3, GCaMP6s, and G-GECO1.0. Videos were obtained using the time series mode in ZEN software at a rate of 10 fps. Quantification of fluorescence intensity was conducted using ZEN software. For drug testing, isoproterenol (10 uM) and verapamil (0.2 uM) were dissolved in DMSO. 0.1% DMSO was used as a control. Recordings were performed 10 minutes after treatment of isoproterenol and 3 minutes after treatment of verapamil.

Lin Y., Liu H., Klein M., Ostrominski J., Hong S.G., Yada R.C., Chen G., Navarengom K., Schwartzbeck R., San H., Yu Z.X., Liu C., Linask K., Beers J., Qiu L., Dunbar C.E., Boehm M, & Zou J. (2018). Efficient differentiation of cardiomyocytes and generation of calcium-sensor reporter lines from nonhuman primate iPSCs. Scientific Reports, 8, 5907.

+ Open protocol

+ Expand

Quantifying Cell Death with siRNA

Check if the same lab product or an alternative is used in the 5 most similar protocols

For initial experiments, cells were transfected with either siDeath or scRNA. Five days later, cells were stained with 5 μg/mL propidium iodide (PI), fixed with 10% formalin, and stained with 750 nM 4’,6-diamidino-2-phenylindole (DAPI). Microscopic images were captured at 5X magnification using a Zeiss Axio observer A1 microscope with a Zeiss Axiocam MRm camera and AxioVision software. Live and dead cells were quantified using ImageJ v1.47 software, and dead cells were subtracted from the live cell count to yield the total cell count for each well. To measure the effect of the functional siRNAs, cells were transfected with scRNA, siYAPl, siNKCCl, siRobol, siEGFR, siSurvivin, or a combination of all five functional sequences. For all groups except the scRNA control, cells were administered 120 nM total functional siRNA. Cell death was measured and quantified as above.

Kozielski K.L., Ruiz-Valls A., Tzeng S.Y., Guerrero-Cázares H., Rui Y., Li Y., Vaughan H.J., Gionet-Gonzales M., Vantucci C., Kim J., Schiapparelli P., Al-Kharboosh R., Quiñones-Hmojosa A, & Green J.J. (2019). Cancer-selective Nanoparticles for Combinatorial siRNA Delivery to Primary Human GBM in vitro and in vivo. Biomaterials, 209, 79-87.

+ Open protocol

+ Expand

Gram-Staining of Frozen Tissue Sections

Check if the same lab product or an alternative is used in the 5 most similar protocols

For bacterial stains frozen model sections (~ 1 cm²) were cut in a cryomicrotome at − 20 °C. Slices were set to measure 5 μm in diameter and then subjected to standard Gram-staining using the Gram stain tissue kit (Sigma, St. Louis, MO, USA). Staining was performed according to the manufacturer’s instructions relying on precooled acetone (− 20 °C, 20 min) as fixation agent. The results were recorded using a standard Axio Observer A1 microscope (Zeiss, Oberkochen, Germany).

Lemoine L., Dieckmann R., Al Dahouk S., Vincze S., Luch A, & Tralau T. (2020). Microbially competent 3D skin: a test system that reveals insight into host–microbe interactions and their potential toxicological impact. Archives of Toxicology, 94(10), 3487-3502.

+ Open protocol

+ Expand

Histological Analysis of Dissected Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice were sacrificed and autopsied, and then dissected tissue samples were fixed for 24 h in 4% paraformaldehyde, dehydrated, and embedded in paraffin. Paraffin blocks were sectioned at 4 µm and stained with H&E. Images were acquired using an Axio Observer A1 microscope (Carl Zeiss).

Viny A.D., Ott C.J., Spitzer B., Rivas M., Meydan C., Papalexi E., Yelin D., Shank K., Reyes J., Chiu A., Romin Y., Boyko V., Thota S., Maciejewski J.P., Melnick A., Bradner J.E, & Levine R.L. (2015). Dose-dependent role of the cohesin complex in normal and malignant hematopoiesis. The Journal of Experimental Medicine, 212(11), 1819-1832.

+ Open protocol

+ Expand

Tissue Fixation and Histological Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice were sacrificed and autopsied, and then dissected tissue samples were fixed for 24 h in 4% paraformaldehyde, dehydrated, and embedded in paraffin. Paraffin blocks were sectioned at 4 μm and stained with haematoxylin and eosin (H&E). Images were acquired using an Axio Observer A1 microscope (Carl Zeiss).

Micol J.B., Pastore A., Inoue D., Duployez N., Kim E., Lee S.C., Durham B.H., Chung Y.R., Cho H., Zhang X.J., Yoshimi A., Krivtsov A., Koche R., Solary E., Sinha A., Preudhomme C, & Abdel-Wahab O. (2017). ASXL2 is essential for haematopoiesis and acts as a haploinsufficient tumour suppressor in leukemia. Nature Communications, 8, 15429.

+ Open protocol

+ Expand

Immunohistochemical Analysis of TA Muscles

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dissected TA muscles were incubated overnight at 4°C in 30% sucrose, flash frozen, cryosectioned at 10 μm, and stored at −80°C prior to staining. Muscle sections were fixed for 3 min in 4% paraformaldehyde (PFA), and if needed, subjected to antigen retrieval: incubation in citrate buffer (10 mM sodium citrate, pH 6.0) in a steamer (Oster #5712) for 15 min after 10 min preheating of buffer (Liu et al., 2015 (link); Tang et al., 2007 (link)). Tissue sections were permeabilized with PBS-T (0.2% Triton X-100) for 10 min and blocked in 10% Normal Goat Serum (NGS; Jackson Immuno Research, West Grove, PA) in PBS-T for 30 min at room temperature. When mouse primary antibodies were used, sections were additionally blocked in 3% AffiniPure Fab fragment goat anti-mouse IgG(H+L) (Jackson Immuno Research) with 2% NGS in PBS at room temperature for 1 hr. Primary antibody incubation in 2% NGS/PBS was carried out at 4°C overnight or 2 hr at RT and sections were incubated with secondary antibodies in 2% NGS/PBS for 1 hr at RT. DAPI staining was used to label nuclei. All slides were mounted with Fluoromount-G (SouthernBiotech, Birmingham, AL). At least four sections from three slides were analyzed per sample. Immunocytochemistry was performed following the same protocol with the exception of the Fab blocking step. Sections and cells were imaged on a Zeiss Axio Observer A.1 microscope (Germany).

Paris N.D., Soroka A., Klose A., Liu W, & Chakkalakal J.V. (2016). Smad4 restricts differentiation to promote expansion of satellite cell derived progenitors during skeletal muscle regeneration. eLife, 5, e19484.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!