Acetylcholinesterase from electrophorus electricus ache

Sodium tetrakis[3,5-bis(trifluoromethyl)phenyl]borate (NaTFPB), calix[4]arene (CX4), 2-nitrophenyl octyl ether (o-NPOE), high-molecular-weight poly(vinyl chloride) (PVC), tetrahydrofuran (THF, inhibitor-free, for HPLC), potassium hydrogen phosphate, potassium dihydrogen phosphate, D-(+)-Glucose monohydrate, potassium chloride, sodium chloride, calcium chloride, magnesium chloride, ammonium chloride, hydrochloric acid (HCl), $\gamma$ -Aminobutyric acid (GABA), L-Glutamic acid monosodium salt hydrate, serotonin, dopamine, Acetylcholinesterase from Electrophorus electricus (AChE), and OmniPur Tris HCl buffer were purchased from Sigma Aldrich. We prepared all stock solutions with MiliQ deionized water (resistivity of 18.2 MΩ·cm) unless stated otherwise. The sheep brain tissue was purchased from a local market (Tehran Market, Los Angeles, CA, USA).

Dulbecco’s modified Eagle medium (DMEM), fetal bovine serum (FBS), trypsin-EDTA, and phosphate-buffered saline (PBS) pH 7.4 were obtained from Lonza (Basel, Switzerland); rIL-6 was produced inhouse as reported [19 (link),20 (link)]. Chemiluminescent HRP substrate kit and centrifugal filter units (Amicon Ultra 10K) were obtained from Millipore (Burlington, MA). Acetylcholinesterase from Electrophorus electricus (AChE) and acetylthiocholine, 5’,5’-dithiobis-2-nitrobenzoic acid (DTNB) were purchased from Sigma-Aldrich.