Animal breeding and killing were performed in accordance to European Commission guidelines and French legislation (2010/63/UE) and procedures were approved by the French Ministry of Research (Agreement # 01479.01). GluD1 knockout,5 (link) GluD2 Hotfoot (HO)40 (link) and DAT-Cre39 (link) mice were genotyped as described.16 (link), 39 (link) Homozygous GluD1-knockout mice were obtained from breeding heterozygous parents. WT mice were purchased from Janvier Labs (Le Genest-Saint-Isle, France). All mice had C57BL/6 background. Whole-cell electrophysiological recordings in midbrain slices from P14–P18 mice, and in vivo juxtacellular recordings in 6–12 week-old anesthetized mice were performed using standard procedures.
Wt mice
Manufactured by Janvier Labs
Sourced in France
WT mice are a type of laboratory mouse that are genetically normal and serve as a control group for experimental studies. They provide a baseline for comparison to genetically modified or treated mice. The core function of WT mice is to establish a reference point for evaluating the effects of specific genetic or environmental factors on biological processes.
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Lab products found in correlation
3 protocols using wt mice
1
Electrophysiological Analysis of Mutant Mice
2
High-Fat Diet Feeding in Mice
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Seven-week-old Wt mice (Janvier Labs, Le Genest-Saint-Isle, France) were acclimated to our animal facilities under a 12-hour/12-hour light/dark cycle at a temperature of 21°C ± 2°C and were fed ad libitum either a HFD (45% fat D12451; Research Diet, New Brunswick, NJ) or control diet (Ctrl D) for 33 weeks.
3
Murine Models for Thrombosis and Lung Injury
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All animal procedures were in accordance with the German animal protection law and the Directive 2010/63/EU of the European Parliament and were approved by the Government of Bavaria (Regierungspräsidium Oberbayern), Munich, Germany. Surgical procedures were carried out under short-term anesthesia using midazolam (5 mg/kg), fentanyl (0.05 mg/kg), and medetomidine hydrochloride (0.5 mg/kg). In vivo experiments were carried out using male animals, for ex vivo experiments both males and females were used. Experiments were carried out using 12–20 weeks old mice. Specifically, all mice undergoing BM transplantation were 20 weeks old at the time of final experiments (thrombus formation in vivo, lung injury model) were carried out. All other mice were 12 weeks old at the time of final experiments. All controls were age- and sex-matched. All mice were on C57Bl/6 background. WT mice were obtained from Janvier Labs (France). Cramp−/−, P-selectin−/−, PF4Cre/+Sykfl/fl, and LysM-GFP mice were described previously3 (link),29 (link),61 (link),62 (link). Mice were maintained in a specific pathogen-free environment and fed standard mouse diet ad libitum.
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