Dca 2000 analyzer

We used standard cut-offs from the literature as described below for
classifying metabolic syndrome and its components (32 (link)). Participants were classified as having elevated
blood pressure if they had systolic blood pressure ≥ 130 mm Hg or
diastolic blood pressure ≥ 85 mm Hg or reported antihypertensive
medication use. Elevated triglycerides were defined as levels ≥ 150
mg/dL or a history of medication use for elevated triglycerides. Low HDL-C was
defined as levels < 40 mg/dL in men and levels < 50 mg/dL in
women or history of drug treatment for reduced HDL-C. Elevated fasting glucose
was defined as levels ≥ 100 mg/dL or a history of medication use for
elevated glucose.
Glucose and insulin levels were evaluated at fasting and after
administration of a 75-g glucose load at 30, 60, and 120 minutes. Glucose was
measured using an enzymatic colorimetric assay. Plasma insulin concentrations
were analyzed using an immunochemiluminometric assay. Insulin resistance was
estimated using HOMA-IR [Fasting glucose × Fasting
insulin/405]. Glycosylated hemoglobin (HbA1C) was measured with an assay
based on a latex immunoagglutination inhibition method (DCA 2000+
Analyzer, Siemens Healthcare Diagnostics, NY, US). Pre-diabetes was defined
using standard cutoffs as fasting plasma glucose between 100–125 mg/dL,
2-hr OGTT 140–199 mg/dL, or HbA1C of 5.7–6.4% (36 ).

All participants were asked to fast for 10 hours prior to the appointment and through the two-hour blood drawing. FPG and insulin levels were determined at baseline and 30, 60, and 120 minutes after administration of a 75-g glucose load, using an enzymatic colorimetric assay. Plasma insulin concentrations were analyzed using an immunochemiluminometric assay, and HOMA-IR was calculated as [FPG (mg/dL) × fasting insulin (μg/dL)]/405]. HbA1c was measured with an assay based on a latex immunoagglutination inhibition method (DCA 2000+ Analyzer, Siemens Healthcare Diagnostics, Tarrytown, NY, USA). Participants were classified as having IFG (FPG 100-125 mg/dl), IGT (2hPG 140-199 mg/dl), impaired glycated hemoglobin (HbA1c 5.7%-6.4%), or normal glucose tolerance (FPG <100 mg/dl, 2hPG <140 mg/dl, and HbA1c <5.7%) following the American Diabetes Association criteria (2011) (link). Pre-diabetes was defined as having at least one of these diagnostic criteria. A modified definition of prediabetes based on the American Diabetes Association glucose thresholds for prediabetes and/or 1hPG concentration >155 mg/dl (Abdul-Ghani et al., 2008 (link)) was also assessed. Since there is no consensus on a cut point to define HOMA-IR, the study population-specific 75^th percentile was used (HOMA-IR ≥3.13).