Experiments were performed with one magnetic and seven types of engineered NPs that were potentially relevant for in vivo exposure. ANATAZ TiO
2 (Sigma-Aldrich 637254) and P25 TiO
2 (Sigma-Aldrich 718467) NPs were obtained from Sigma (St Luis, MO, USA), DrA TiO
2 (Dr. Adorable Inc, 712392053501) from Dr. Adorable Inc (Chicago, Illinois, USA), N.B. TiO
2 (Naturally Balmy Ltd., NB-2257-1) from Naturally Balmy Ltd. (NB-2257-1, Bournemouth, UK), silver NPs (Biopure 20 nm) were obtained from nanoComposix (San Diego, CA, USA), and engineered SiO
2 dispersed in 2-propanol were obtained from Nanotesla Institute Ljubljana (Ljubljana, Slovenia). Food-grade (FG) TiO
2 NPs were obtained from Cake Stuff (Glasgow, UK). All TiO
2 NP powders were temperature-sterilized, dispersed in ultrapure water, and sonicated.
Biomedical polyacrylic acid (PAA)-coated cobalt ferrite NPs were prepared as described previously [51 (
link),105 (
link)]. Briefly, cobalt ferrite (CoFe
2O
4 and Co-ferrite) NPs cores were prepared using the coprecipitation method and stabilized in water. The NP-cores were coated in situ with a 45% (m/m) water solution of polyacrylic acid and sodium salt (PAA) with a molecular weight of 8 kDa (Sigma-Aldrich). The NPs were dialyzed against distilled water and sterilized via filtration using a 0.22 mm pore size syringe filter (Techno Plastic Products TPP, Trasadingen, Switzerland).
Pavlin M., Lojk J., Strojan K., Hafner-Bratkovič I., Jerala R., Leonardi A., Križaj I., Drnovšek N., Novak S., Veranič P, & Bregar V.B. (2022). The Relevance of Physico-Chemical Properties and Protein Corona for Evaluation of Nanoparticles Immunotoxicity—In Vitro Correlation Analysis on THP-1 Macrophages. International Journal of Molecular Sciences, 23(11), 6197.