Immunoglobulin G and M, transferrin, haptoglobin, alpha-2-macroglobulin, alpha-1-antitrypsin, complement C3, and alpha-1-acid glycoprotein purified from human serum were purchased from Sigma-Aldrich (St. Louis, MO). Immunoglobulin A purified from human plasma was purchased from CalBiochem (La Jolla, CA). Anti-human-IgA (α-chain specific)-agarose and anti-human-IgM (μ-chain specific)-agarose used for protein enrichment were purchased from Sigma-Aldrich (St. Louis, MO). Sequencing grade modified trypsin and dithiothreitol (DTT) were purchased from Promega (Madison, WI). Pronase E from Streptomyces griseus and iodoacetamide (IAA) were purchased from Sigma-Aldrich (St. Louis, MO).
Alpha 1 acid glycoprotein
Manufactured by Merck Group
Sourced in Germany
Alpha-1-acid glycoprotein is a laboratory reagent used in various analytical and research applications. It is a plasma protein that acts as an acute-phase reactant, with its levels increasing in response to inflammation or infection. The core function of alpha-1-acid glycoprotein is to bind and transport a wide range of endogenous and exogenous compounds, including drugs, hormones, and other small molecules.
Automatically generated - may contain errors
Lab products found in correlation
Haptoglobin, by Merck Group (2 mentions)
Alpha 2 macroglobulin, by Merck Group (2 mentions)
Alpha 1 antitrypsin, by Merck Group (2 mentions)
Transferrin, by Merck Group (2 mentions)
Human serum albumin (hsa), by Merck Group (2 mentions)
Complement c3, by Merck Group (1 mentions)
Dithiothreitol (dtt), by Promega (1 mentions)
Pronase e from streptomyces griseus, by Merck Group (1 mentions)
Iodoacetamide iaa, by Merck Group (1 mentions)
Sequencing grade modified trypsin, by Promega (1 mentions)
Sulfuric acid, by ITW Reagents (1 mentions)
Disodium hydrogen phosphate, by ITW Reagents (1 mentions)
Sodium dihydrogen phosphate, by ITW Reagents (1 mentions)
Dopamine, by Merck Group (1 mentions)
Sodium chloride (nacl), by ITW Reagents (1 mentions)
Hydrochloric acid (hcl), by ITW Reagents (1 mentions)
Microcrystalline cellulose powder, by Merck Group (1 mentions)
Uric acid, by Merck Group (1 mentions)
Ultraclear device, by Siemens (1 mentions)
L tyrosine, by Merck Group (1 mentions)
5 protocols using alpha 1 acid glycoprotein
1
Purification and Characterization of Human Serum Proteins
2
Glycoprotein Profiling by HILIC
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A uniform mixture of six standard glycoproteins was used as the starting material, including IgG (56834, catalog numeber), IgA (I4036), IgM (I8260), Alpha-1-acid glycoprotein (G9885), Alpha-2-macroglobulin (M6159) and Haptoglobin (H3536) (Sigma). Trypsinization and HILIC enrichment were performed as previously reported22 (link).
3
Synthesis and Characterization of CNC/SWCNT Hybrids
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K4Fe(CN)6·3H2O, K3Fe(CN)6, Ru(NH3)6Cl3, N,N-dimethylformamide (DMF), alpha-1-acid glycoprotein (AGP) (≥ 99%), transferrin (≥ 98%), human serum (certified reference material, ERM-DA470K/IFCC), potassium osmate (VI) dihydrate, N,N,N′,N′-tetramethylenediamine (TEMED), potassium nitrate (≥ 98%), uric acid, dopamine, l -tyrosine, and cellulose microcrystalline powder (20 µm) were purchased from Sigma–Aldrich (Darmstadt, Germany). Hydrochloric acid (37%), sulfuric acid (95–98%), sodium chloride, disodium hydrogen phosphate, and sodium dihydrogen phosphate were purchased from PanReac (Barcelona, Spain).
SWCNTs were acquired from Carbon Solutions Inc. (type P2-SWNT, Riverside, CA, USA). These correspond to a purified material following a non-specified non-oxidative process.
For the synthesis of CNC and characterization of CNC/SW hybrids, ultrapure water was obtained from a Siemens Ultraclear device, with a conductivity of 0.055 μS cm−1. For the analytical studies, all aqueous solutions were prepared in Milli-Q water (Merck Millipore, Darmstadt, Germany).
SWCNTs were acquired from Carbon Solutions Inc. (type P2-SWNT, Riverside, CA, USA). These correspond to a purified material following a non-specified non-oxidative process.
For the synthesis of CNC and characterization of CNC/SW hybrids, ultrapure water was obtained from a Siemens Ultraclear device, with a conductivity of 0.055 μS cm−1. For the analytical studies, all aqueous solutions were prepared in Milli-Q water (Merck Millipore, Darmstadt, Germany).
4
Biophysical Characterization of Drug-Protein Interactions
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Temozolomide (TMZ) was obtained from Tokyo Chemical Industry, TCI (Tokyo, JP). Human serum albumin (HSA) and alpha-1-acid glycoprotein (AGP) were purchased from Sigma Aldrich (St. Louis, MO). The synthetic phospholipids 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), egg 1,2-diacyl-sn-glycero-3-phosphocholine (EPC) and cholesterol (Chol) were from Sigma-Aldrich (St. Louis, MO), and were used as received. The fluorescent probes 2-dimethylamino-6-lauroylnaphtalene (Laurdan) and 1-6-diphenylhexatriene (DPH) were obtained from Molecular Probes (Eugene, OR), and dissolved in ethanol (3.77 × 10−4 M) and dimethylformamide (DMF) (1 × 10−3 M), respectively. Water was twice distilled in all-glass apparatus and deionized using Milli-Q equipment (Millipore, Madrid, ES). A phosphate buffer (50 mM, 0.1 M NaCl, pH 7.4) and an acetate buffer (100 mM, pH 5.0) were prepared with deionized doubly distilled water. All other compounds were of analytical or spectroscopic reagent grade.
5
Protein Purification and Glycosylation Analysis
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Organic solvents of HPLC grade,
sodium chloride, hydrogen chloride, piperazine, bis-tris-propane,
and 1,4-dimethylpiperazine at highest available purity were purchased
from Sigma-Aldrich GmbH (Taufkirchen, Germany). Ribonucleases from
bovine pancreas (CAS-No 9001-99-4)—A with purity above 90%
and B with purity above 80%—as well as purified proteins from
human plasma (HSA, IgG, alpha-1-antitrypsin, haptoglobin, alpha-1-acid
glycoprotein) were also purchased from Sigma-Aldrich GmbH at the highest
available purity. Alpha-1-acid glycoprotein from bovine plasma with
99% purity was purchased from the same vendor.
Phosphate-buffered
saline (10× PBS), pH 6.6, was prepared in-house. Igepal-CA630,
dithiothreitol, 2-methylpiridine borane complex, procainamide hydrochloride
(ProA), and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich,
St. Louis, Missouri, USA. Glacial acetic acid was produced by Merck,
Darmstadt, Germany; acetonitrile (ACN, LC-MS grade) was from Honeywell,
USA; sodium dodecyl sulfate (SDS) was from Invitrogen, Carlsbad, California,
USA. Peptide-N-glycosidase F (PNGase F) was from
Promega, Madison, Wisconsin, USA.
sodium chloride, hydrogen chloride, piperazine, bis-tris-propane,
and 1,4-dimethylpiperazine at highest available purity were purchased
from Sigma-Aldrich GmbH (Taufkirchen, Germany). Ribonucleases from
bovine pancreas (CAS-No 9001-99-4)—A with purity above 90%
and B with purity above 80%—as well as purified proteins from
human plasma (HSA, IgG, alpha-1-antitrypsin, haptoglobin, alpha-1-acid
glycoprotein) were also purchased from Sigma-Aldrich GmbH at the highest
available purity. Alpha-1-acid glycoprotein from bovine plasma with
99% purity was purchased from the same vendor.
Phosphate-buffered
saline (10× PBS), pH 6.6, was prepared in-house. Igepal-CA630,
dithiothreitol, 2-methylpiridine borane complex, procainamide hydrochloride
(ProA), and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich,
St. Louis, Missouri, USA. Glacial acetic acid was produced by Merck,
Darmstadt, Germany; acetonitrile (ACN, LC-MS grade) was from Honeywell,
USA; sodium dodecyl sulfate (SDS) was from Invitrogen, Carlsbad, California,
USA. Peptide-N-glycosidase F (PNGase F) was from
Promega, Madison, Wisconsin, USA.
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