M100907

Manufactured by Merck Group

Sourced in United States, Israel, Poland, United Kingdom

The M100907 is a laboratory instrument designed for general use in research and analytical applications. It is a versatile piece of equipment that can perform a variety of tasks without any specific intended use mentioned.

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Lab products found in correlation

14 protocols using m100907

Preparation of Serotonin, Capsaicin, and Associated Compounds

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Serotonin hydrochloride (5HT; Sigma–Aldrich, St. Louis, MO) was dissolved in double-distilled water and diluted in 0.9% sterile saline or Hank’s balanced salt solution (HBSS) buffer immediately prior to each use. Capsaicin (CAP; Sigma–Aldrich, St. Louis, MO) was dissolved in 100% ethanol in a fume hood and aliquots were stored at −20 °C as 100 mM stocks. Capsaicin was freshly diluted in 0.9% saline or HBSS buffer prior to each use. The 5HT_2A antagonist, M100907 (Sigma–Aldrich), was dissolved in 20% dimethyl sulfoxide (DMSO) in 0.9% sterile saline, stored as a 2 mM stock solution at 4 °C, and then serial diluted the day of use to a final working solution of 2 nM, 10 nM, or 30 nM M100907 (6% DMSO in 0.9% sterile saline). β-Estradiol (E2; Sigma–Aldrich, St. Louis, MO) was dissolved in 100% ethanol to create a 10 mM stock solution that was further diluted in HBSS buffer for a working solution of 50 nM. Immediately prior to use, complete Freund’s adjuvant (CFA; Sigma–Aldrich) was dissolved 1:1 in 0.9% sterile saline.

Kaur S., McDonald H., Tongkhuya S., Lopez C.M., Ananth S., Hickman T.M, & Averitt D.L. (2021). Estrogen exacerbates the nociceptive effects of peripheral serotonin on rat trigeminal sensory neurons. Neurobiology of Pain, 10, 100073.

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Chemical Reagents and Compounds

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M100907, ketanserin, spiperone, SB204741, bosentan, losartan, prazosin, BAPTA-AM, 2-APB, and Y27632 were obtained from Sigma Chemical Co. (St Louis, MO). All other chemicals and reagents were analytical grade.

, & Zimering M.B. (2018). Circulating Neurotoxic 5-HT2A Receptor Agonist Autoantibodies in Adult Type 2 Diabetes with Parkinson’s Disease. Journal of endocrinology and diabetes, 5(2), 10.15226/2374-6890/5/2/01102.

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Pharmacological Agents for Cellular Assays

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M100907, ketanserin, spiperone, DOI, bosentan, losartan, prazosin, U73211, BAPTA-AM, 2-APB were obtained from Sigma Chemical Co. (St Louis, MO.) All other chemicals and reagents were analytical grade.

, & Zimering M.B. (2017). Diabetes Autoantibodies Mediate Neural- and Endothelial Cell- Inhibitory Effects Via 5-Hydroxytryptamine- 2 Receptor Coupled to Phospholipase C/Inositol Triphosphate/Ca2+ Pathway. Journal of endocrinology and diabetes, 4(4), 10.15226/2374-6890/4/4/00184.

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Cognitive Flexibility: M100907 Effects

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In Experiment 2, all mice received an injection of 10% DMSO sterile water prior to acquisition. Mice received an IP injection twenty four hours after acquisition testing of either 10% DMSO sterile water, 0.01, or 0.1 mg/kg of M100907 [Sigma Aldrich, St Louis, MO]. M100907 was mixed in 10% DMSO sterile water. Thirty minutes after an injection, mice began retention trials followed immediately by the reversal learning test. The acquisition test session ranged from 20 to 91 min. The reversal learning session ranged from 33 to 110 min. All mice in this experiment reached acquisition and reversal learning criterion. Thus, no mice were excluded from the behavioral analyses.

Amodeo D.A., Jones J.H., Sweeney J.A, & Ragozzino M.E. (2014). Risperidone and the 5-HT2A receptor antagonist, M100907 improve probabilistic reversal learning in BTBR T+ tf/J mice. Autism research : official journal of the International Society for Autism Research, 7(5), 555-567.

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Serotonergic Modulation in Rat Behavior

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Rats were tested in 2 cohorts. With the exception of WAY 100635, which was administered subcutaneously, all drugs targeting the serotonergic system were injected i.p.. Cohort 1 (n = 16) received Citalopram hydrobromide (selective serotonin reuptake inhibitor), M100907 (5-HT2A receptor antagonist), and SB242,084 (5-HT2C receptor antagonist). Citalopram (Tocris Bioscience) was prepared in 0.9% saline. M100907 (Sigma Aldrich) was prepared in 0.9% saline with the pH adjusted to 6.5 with acetic acid. The vehicle for SB 242,084 (Tocris Bioscience) consisted of 240 mg of anhydrous citric acid and 4 g of cyclodextrine dissolved in 50 mL of 0.9% saline to yield an 8% cyclodextrin in 25 mM citric acid solution. The pH of this solution was then adjusted to 6.4 by adding 5 mL of 0.1 M NaOH. Cohort 2 (n = 18) received WAY-100635 (5-HT1A receptor antagonist) followed by 8-OH-DPAT (5-HT1A receptor agonist). WAY-100635 (Sigma Aldrich) was prepared in phosphate-buffered saline (PBS). 8-OH-DPAT (Sigma Aldrich) was prepared in 0.9% saline. The drug doses and timing of injections prior to testing were based on previous studies that used these drugs in comparable behavioral tests (Winstanley et al. 2004 (link)); (Bari et al. 2009 (link)); (Boulougouris, Glennon and Robbins 2008 (link), Zeeb et al. 2009 (link)).

Blaes S.L., Orsini C.A., Mitchell M.R., Spurell M.S., Betzhold S.M., Vera K., Bizon J.L, & Setlow B. (2018). Monoaminergic modulation of decision making under risk of punishment in a rat model. Behavioural pharmacology, 29(8), 745-761.

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Acute Neuropharmacological Interventions

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M100907 (MDL-100907, Volinanserin, (R)-(+)-a-(2,3-dimethoxyphenyl)-1-[2-(4-fluorophenyl)ethyl]-4-pipidinemethanol) (Sigma-Aldrich, St. Louis, USA) was dissolved in 0.9% normal saline and administered intraperitoneally in a single, acute dose of 0.003, 0.01, or 0.1 mg/kg 15 minutes before behavioral testing.
DOI ((±)-2,5-Dimethoxy-4-iodoamphetamine hydrochloride) (Sigma-Aldrich, St. Louis, USA) was dissolved in normal saline and administered intraperitoneally in a single, acute dose of 3.0 mg/kg.
RS-102221 (4-dione hydrochloride hydrate) (Sigma-Aldrich, St. Louis, USA) was dissolved in 0.9% normal saline and administered intraperitoneally in a single, acute dose of 2.0 mg/kg.

Panzini C.M., Ehlinger D.G., Alchahin A.M., Guo Y, & Commons K.G. (2017). 16p11.2 Deletion Syndrome Mice Perseverate with Active Coping Response to Acute Stress - Rescue by Blocking 5-HT2A Receptors. Journal of neurochemistry, 143(6), 708-721.

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Pharmacological Manipulation of Psychoactive Substances

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Ketamine hydrochloride (ketamine, Tocris, 3131), R,R-HNK (Tocris, 6094), (+)-LSD (Sigma-Aldrich, L7007), PSI (Biosynth Carbosynth, P-7800), lisuride maleate (Tocris, 4052), cabergoline (Sigma-Aldrich, C0246), ketanserin (+)-tartrate (ketanserin, Sigma-Aldrich, S006) and M100907 (Sigma-Aldrich, M3324) were solubilized in dimethyl sulfoxide (DMSO) and diluted 1:1,000 (final concentration 0.1% DMSO) for in vitro experiments. Drugs were diluted in sterile 0.9% saline and injected intraperitoneally (10 ml kg⁻¹) for in vivo experiments. All controlled psychotropic substances were obtained, stored, handled and disposed of as regulated under the permit issued by the Finnish Medicines Agency to the Faculty of Medicine, Medicum (FIMEA/2022/000945).

Moliner R., Girych M., Brunello C.A., Kovaleva V., Biojone C., Enkavi G., Antenucci L., Kot E.F., Goncharuk S.A., Kaurinkoski K., Kuutti M., Fred S.M., Elsilä L.V., Sakson S., Cannarozzo C., Diniz C.R., Seiffert N., Rubiolo A., Haapaniemi H., Meshi E., Nagaeva E., Öhman T., Róg T., Kankuri E., Vilar M., Varjosalo M., Korpi E.R., Permi P., Mineev K.S., Saarma M., Vattulainen I., Casarotto P.C, & Castrén E. (2023). Psychedelics promote plasticity by directly binding to BDNF receptor TrkB. Nature Neuroscience, 26(6), 1032-1041.

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Psilocybin and Pharmacological Agents Protocol

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PSIL was supplied by Usona Institute, Madison, WI, USA and was determined by AUC at 269.00 nm (UPLC) to contain 98.75% psilocybin and stored in a cool light-sealed safe. 5-HTP (5-Hydroxytryptophan), M100907 ((R)-(+)-α-(2,3-dimethoxyphenyl)-1-[2-(4-fluorophenyl)ethyl]-4-pipidinemethanol), 8-OH-DPAT((±)-8-Hydroxy-2-(dipropylamino)tetralin hydrobromide) and EPPTB (N-(3-Ethoxy-phenyl)-4-pyrrolidin-1-yl-3-trifluoromethyl-benzamide) were purchased from Sigma-Aldrich, Tel Aviv, Israel. RS-102221 (8-[5-(2,4-Dimethoxy-5-(4-trifluoromethylphenylsulphonamido)phenyl-5-oxopentyl]-1,3,8-triazaspiro[4.5]decane-2,4-dione hydrochloride) was purchased from Biotest, Kfar Saba, Israel. 5-HTP, M100907 and EPPTB were stored in 4 °C. 8-OH-DPAT and RS102221 were stored in room temperature. PSIL was dissolved in 100% saline (0.9% NaCl) solution. 5-HTP, M100907, 8-OH-DPAT and RS-10222 were dissolved in 5% DMSO + 95% saline (0.9% NaCl) solution. EPPTB was dissolved in 5% ethanol + 5% kolliphor + 90% saline (0.9% NaCl) solution. All solutions were prepared to the appropriate concentration of 10 µL/g for administration (by intraperitoneal (i.p.) injection). Vehicle-treated condition represents injection of the appropriate solvent and the equivalent volume of the drugs administered. Fresh injectable solutions of the compounds were prepared for each experiment.

Shahar O., Botvinnik A., Esh-Zuntz N., Brownstien M., Wolf R., Lotan A., Wolf G., Lerer B, & Lifschytz T. (2022). Role of 5-HT2A, 5-HT2C, 5-HT1A and TAAR1 Receptors in the Head Twitch Response Induced by 5-Hydroxytryptophan and Psilocybin: Translational Implications. International Journal of Molecular Sciences, 23(22), 14148.

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Quantification of 25I-NBOMe Using HPLC

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4-Iodo-2,5-dimethoxy-N-(2-methoxybenzyl)phenethylamine hydrochloride (25I-NBOMe) was purchased from Chiron AS (Trondheim, Norway). All chemicals used for high-performance liquid chromatography (HPLC) were obtained from Merck (Warszawa, Poland), O-phthalaldehyde (OPA) was from Sigma-Aldrich. Ketamine hydrochloride and xylazine hydrochloride were from Biowet (Pulawy, Poland). M100907, SB242084 dihydrochloride hydrate and WAY100635 maleate salt were from Sigma-Aldrich (Poznan, Poland).

Herian M., Wojtas A., Sobocińska M.K., Skawski M., González-Marín A, & Gołembiowska K. (2020). Contribution of serotonin receptor subtypes to hallucinogenic activity of 25I-NBOMe and to its effect on neurotransmission. Pharmacological Reports, 72(6), 1593-1603.

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Serotonin Receptor Antagonist Modulation

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Serotonin hydrochloride (Sigma) was resuspended in water to a stock concentration of 100 mM and sterile-filtered. M100907 (volinanserin) (Sigma-Merck), a specific 5-HT 2A receptor antagonist, was resuspended in DMSO to a stock concentration of 10 mM.
Cells were treated with either 100 μM serotonin alone, or with a combination of 10 μM M100907 and 100 μM serotonin, or with 1:1000 DMSO and 100 μM serotonin for 6 days. Medium was refreshed after 3 days.

Cardon I., Grobecker S., Jenne F., Jahner T., Rupprecht R., Milenkovic V.M, & Wetzel C.H. (2024). Serotonin effects on human iPSC-derived neural cell functions: from mitochondria to depression. Molecular psychiatry.

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