Cd27 bv786

Manufactured by BD

CD27-BV786 is a fluorochrome-conjugated antibody that binds to the CD27 antigen, a member of the tumor necrosis factor receptor superfamily. It is used for the detection and analysis of CD27-expressing cells in flow cytometry applications.

Automatically generated - may contain errors

Lab products found in correlation

3 protocols using cd27 bv786

Multiparameter Flow Cytometry of CSF Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Flow cytometry was conducted using an LSRFortessa (BD Biosciences). A panel consisting of antibodies conjugated to six different fluorophores was used to classify subsets of memory T cells and for drop-seq. Antibodies used were: CD8α-Pacific blue (BioLegend), CD3-BV650 (BD Biosciences), CD45RA-APC-Cy7 (BioLegend), CCR7–488 (Bio-Legend), IL-7Rα-PE (BioLegend) and CD27-PE-Cy7 (BioLegend). For characterization of CSF cells, this same panel was used, but CD19-PE-Cy5 (BioLegend) and CD14-Qdot-705 (Thermo Fisher Scientific) were included. For sorting CSF T cells for TCR plate-seq, the following antibodies were used: CD8α-PE (BioLegend), CD161-PE-Cy7 (BioLegend), CXCR3-APC (BioLegend), CD4-APC-Alexa700 (Thermo Fisher Scientific), CD39-APC-Cy7 (BioLegend), CD38-FITC (BioLegend), PD-1-BV421 (BD Biosciences), CD45RA-BV605 (BD Biosciences), CD3-BV650 (BD Biosciences), CD27-BV786 (BD Biosciences) and CD127-BUV395 (BD Biosciences). For each experiment, a compensation matrix was developed using singly stained and unstained controls or fluorescent beads, and all analysis was conducted in Cytobank.

Gate D., Saligrama N., Leventhal O., Yang A.C., Unger M.S., Middeldorp J., Chen K., Lehallier B., Channappa D., De Los Santos M.B., McBride A., Pluvinage J., Elahi F., Tam G.K., Kim Y., Greicius M., Wagner A.D., Aigner L., Galasko D.R., Davis M.M, & Wyss-Coray T. (2020). Clonally expanded CD8 T cells patrol the cerebrospinal fluid in Alzheimer’s disease. Nature, 577(7790), 399-404.

+ Open protocol

+ Expand

Comprehensive Vδ2 T Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

On day 14 of expansion, Vδ2 T cells were collected and washed in PBS and stained with Aqua viability dye (ThermoFisher). Next, cells were surface stained for the following antibodies: CD26 FITC (BA5b, Biolegend), CD45RA PerCpCy5.5 (HI100; Biolegend), CD160 Alexa Fluor 647 (BY55; BD Biosciences), TIGIT APC Fire 750 (A15153G; Biolegend), PD‐1 BV421 (EH12.2H7; Biolegend), NKG2D BV650 (1D11; BD Biosciences), CD27 BV786 (L128; BD Biosciences), CD94 BUV395 (HP‐3D9; BD Biosciences), Tim‐3 BUV737 (7D3; BD Biosciences), CD3 BUV805 (SK7; BD Biosciences), Vδ2 PE (B6; Biolegend) and 2B4 PE‐Dazzle (C1.7; Biolegend). After surface staining, cells were washed and resuspended in PBS containing 2% FCS before acquisition on a BD LSR Fortessa using BD FACS Diva.

Field K.R., Wragg K.M., Kent S.J., Lee W.S, & Juno J.A. (2024). γδ T cells mediate robust anti‐HIV functions during antiretroviral therapy regardless of immune checkpoint expression. Clinical & Translational Immunology, 13(2), e1486.

+ Open protocol

+ Expand

Multiparameter Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

All antibodies used in this study are anti-human specific monoclonal antibodies. CD3-Pacific Orange (Clone OKT3) and CD19-Alexa488 (Clone BU12) were produced in-house at DRFZ. CD19-PE/Cy7 (Clone HIB19, eBioscience, Cat. #25-0199-42, RRID: AB_1582278), CD27-BV786 (Clone L128, BD Biosciences, Cat. #563327, RRID: AB_2744353), CD38-APC/Cy7 (Clone HIT2, Biolegend, Cat. #303534, RRID: AB_2561605), IRF4-PerCP/Cy5.5 (Clone IRF4.3E4, Biolegend, Cat. #646415, RRID: AB_2728481), BLIMP1/PRDM1-APC (Clone 646702, R&D Systems, Cat. #IC36081A, RRID: AB_11128645), XBP-1S-BV421 (Clone Q3-695, BD Biosciences, Cat. #563382, RRID: AB_2738168), β2M-APC (Clone 2M2, Biolegend, Cat. #316312, RRID: AB_10641281), Mouse IgG1 κ Isotype Control-APC (Clone MOPC-21, Biolegend, Cat. #400121, RRID: AB_326443), Mouse IgG1, κ Isotype Control-BV421 (Clone X40, BD Biosciences, Cat. #562438, RRID: AB_11207319), Rat IgG1, κ Isotype Control-PerCP/Cy5.5 (Clone RTK2071, Biolegend, Cat. #400425, RRID: AB_893689).

Le T.A., Chu V.T., Lino A.C., Schrezenmeier E., Kressler C., Hamo D., Rajewsky K., Dörner T, & Dang V.D. (2022). Efficient CRISPR-Cas9-mediated mutagenesis in primary human B cells for identifying plasma cell regulators. Molecular Therapy. Nucleic Acids, 30, 621-632.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!