Serum blood samples were obtained from the children after an 8 h overnight fast and were immediately centrifuged and stored in −70 °C [40 (link)]. Following slow thawing: (i) Serum leptin samples were processed with a leptin ELISA kit (Biovendor 62100 Brno Czech Republic) with an intra assay variation CV% of 4.2%, inter assay variation CV% 6.7%, and a detection limit 0.2 ng/mL; (ii) adiponectin serum samples were processed with an ELISA kit (Invitrogen, Campus Vienna Biocenter 2, 1030,Vienna, Austria), with an intra assay variation CV% of 4.2%, an inter assay variation CV% of 3.1%, and a detection limit: <0.012 ng/mL; (iii) the hs-CRP serum samples were obtained via the use of a hs-CRP ELISA kit (HyCult Biotech, Frontstraat 2A, 5405 PB Uden, Netherlands), with intra assay variation CV% 4.1%, with an inter assay variation CV% of 6.3%, and a detection limit < 0.4 ng/mL; and (iv) for measuring the IL-6 levels an IL6 ELISA kit (Origene Tech., 9620 Medical Center Drive, 200, Rockville, MA, USA) was used with intra assay variation CV% 6.2%, inter assay variation CV% 7.2%, and a detection limit < 0.3 pg/mL [40 (link)].
Leptin elisa kit
Manufactured by BioVendor
Sourced in United States
The Leptin ELISA kit is a laboratory assay used to quantify the levels of the hormone leptin in biological samples. The kit employs the enzyme-linked immunosorbent assay (ELISA) technique to detect and measure the concentration of leptin. This product is designed for research use only and its performance characteristics have been validated.
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Lab products found in correlation
5 protocols using leptin elisa kit
1
Measuring Inflammatory Biomarkers in Children
2
Plasma Biomarker Evaluation Protocol
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Containers, including blood and heparin, were inverted and left to stand. The plasma was obtained from the filtrate after centrifugal separation at 3,000 rpm for 15 min. Plasma concentrations of TGs, cholesterol, and leptin were measured by using the Triglyceride E-test Wako kit (Wako Pure Chemical Corporation, Osaka, Japan), Cholesterol E-test Wako kit (Wako Pure Chemical Corporation, Osaka, Japan), and leptin ELISA kit (Bio Vendor Laboratory Medicine, Inc., Brno-Řečkovice a Mokrá Hora, Czechia;Number: RD291001200R; Lot: E19-024), respectively.
3
Comprehensive Blood Lipid Profiling
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Blood lipid analysis was performed to determine the levels of TG, TC, HDL-C, and LDL-C. Blood samples (10 ml) were collected by a registered nurse from the antecubital vein and then centrifuged using a Combi-514R centrifuge (Hanil, Korea) at 3000 rpm for 10 min before analysis. Insulin resistance was calculated using the HOMA-IR method based on the following formula [24 (link)]: HOMA-IR = [fasting plasma insulin (Uu/ml) × fasting plasma glucose (ml/dl)] / 405. Leptin levels were measured from the separated serum by using a leptin ELISA kit DSL (USA), and resistin was analyzed using an antibody enzyme-linked immunoassay kit (Bio Vendor Laboratory Medicine, USA) and subjected to an enzyme-linked immunosorbent assay (ELISA, Hitached 747 analyzer, Tokyo, Japan) [25 ]. All measurements, excluding physical activity levels, were taken after maintaining a fasting period of at least 8 hours. Measurements were conducted between 8 and 10 a.m. with participants in a state of metal removal and light clothing.
4
Metabolic Parameter Changes in Offspring
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To assess changes in metabolic parameters at 2 time points (3 weeks and 6 months of age), the offspring of each group were fasted overnight, and blood was collected via cardiac puncture into heparinized tubes after rapid decapitation. Plasma glucose, triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) levels were analyzed by an enzymatic colorimetric method using a Cobas 8000 instrument (Roche Diagnostics GmbH, Mannheim, Germany). Insulin and leptin levels were also assayed using a rat insulin enzyme-linked immunosorbent assay (ELISA) kit (Alpco, Salem, NH, USA) and a leptin ELISA kit (BioVendor, Modrice, Czech Republic), respectively, according to the manufacturers' instructions.
5
Measuring Serum Biomarkers via ELISA
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After careful and slow thawing: (i) Serum leptin samples were processed with a leptin ELISA kit (Biovendor 62100, Brno, Czech Republic), with an intra Assay Variation CV% of 4.2%, an inter Assay Variation CV% of 6.7%, and a detection Limit of 0.2 ng/mL; (ii) Adiponectin serum samples were processed with an ELISA kit (Invitrogen, Campus Vienna Biocenter 2, 1030, Vienna, Austria), with an intra Assay Variation CV% of 4.2%, an inter Assay Variation CV% of 3.1%, and a detection Limit of 0.012 ng/mL; (iii) an hs-CRP ELISA kit (HyCult Biotech, Frontstraat 2A, 5405 PB Uden) was used for hs-CRP serum samples, with an intra Assay Variation CV% of 4.1%, an inter Assay Variation CV% of 6.3%, and a detection limit of 0.4 ng/mL; and (iv) an IL-6 ELISA kit (Origene Tech., 9620 Medical Center Drive, 200, Rockville, MD, USA) with an intra Assay Variation CV% of 6.2%, an inter Assay Variation CV% of 7.2%, and a detection Limit of 0.3 pg/mL were used for IL-6 levels.
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